Molecular Biotechnology

, Volume 34, Issue 2, pp 225–237

Large-Scale transfection of mammalian cells for the fast production of recombinant protein


DOI: 10.1385/MB:34:2:225

Cite this article as:
Pham, P.L., Kamen, A. & Durocher, Y. Mol Biotechnol (2006) 34: 225. doi:10.1385/MB:34:2:225


Recombinant proteins (r-proteins) are increasingly important in fundamental research and for clinical applications. As many of these r-proteins are of human or animal origin, cultivated mammalian cells are the host of choice to ensure their functional folding and proper posttranslational modifications. Large-scale transfection of human embryonic kidney 293 or Chinese hamster ovary cells is now an established technology that can be used in the production of hundreds of milligram to gram quantities of a r-protein in less than 1 mo from cloning of its cDNA. This chapter aims to provide an overview of large-scale transfection technology with a particular emphasis on calcium phosphate and polyethylenimine-mediated gene transfer.

Index Entries

HEK 293 CHO COS EBNA1 polyethylenimine suspension serum-free gene transfer 

Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  1. 1.Laboratoire de Biotechnologie Vétérinaire et Alimentaire, Faculté de Médecine VétérinaireUniversité de MontréalQuébecSainte-HyacintheCanada
  2. 2.Biotechnology Research InstituteNational Research Council CanadaMontrealCanada

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