Molecular Biotechnology

, Volume 34, Issue 1, pp 51–54 | Cite as

Enhanced DNA transfer into fish bone cells using polyethylenimine

  • Daniel Braga
  • Vincent Laizé
  • Daniel M. Tiago
  • M. Leonor Cancela
Research Protocols

Abstract

The use of in vitro cell culture systems to assess gene function largely depends on the successful transfer of DNA into target cells. Well developed in mammals, transfection methods are still to be optimized for non-mammalian cell culture systems, like fish. Here we describe a rapid, cost-efficient, and successful method to transfer DNA into a fish bone-derived cell line using polyethylenimine (PEI) as the DNA carrier. Using this method, DNA transfer was remarkably enhanced in comparison with commercially available reagents, as demonstrated by the increased activity of both luciferase and green fluorescent protein observed in the transfected cells. Its efficiency in transferring DNA intoa wide range of cell types, including non-mammalian and hard-to-transfect cells, in addition to a low cost, show that PEI is a reagent of choice for nonviral vector transfection.

Index entries

DNA transfer cell transfection polyethylenimine (PEI) non-mammalian cells 

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Copyright information

© Humana Press Inc 2006

Authors and Affiliations

  • Daniel Braga
    • 1
  • Vincent Laizé
    • 1
  • Daniel M. Tiago
    • 1
  • M. Leonor Cancela
    • 1
  1. 1.Centre of Marine Sciences (CCMAR)University of AlgarveFaroPortugal

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