Molecular Biotechnology

, Volume 30, Issue 2, pp 167–169 | Cite as

A PCR-based method, with internal control, for the detection of Banana bunchy top virus in banana

  • Shahid Mansoor
  • Javaria Qazi
  • Imran Amin
  • Abdullah Khatri
  • Imtiaz A. Khan
  • Saboohi Raza
  • Yusuf Zafar
  • Rob W. Briddon
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Abstract

Banana bunchy top disease is a major constraint to banana production in most regions where this crop is grown. The disease is caused by Banana bunchy top virus (BBTV), a multicomponent, single-stranded DNA virus of the family Nanoviridae. We have designed primers to a conserved region of the master replication-associated protein that are useful for the polymerase chain reaction (PCR)-mediated detection of BBTV. In addition, primers to banana genomic sequence are used as an internal control, overcoming the uncertainty (owing to false-negatives) inherent in PCR diagnostics. Together these primer sets are a valuable tool in the effort to control BBTV, particularly in screening micropropagated banana plantlets for the absence of virus before release to farmers.

Index Entries

Nanovirus diagnostics banana PCR banana bunchy top disease 

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Copyright information

© Humana Press Inc. 2005

Authors and Affiliations

  • Shahid Mansoor
  • Javaria Qazi
  • Imran Amin
  • Abdullah Khatri
    • 2
  • Imtiaz A. Khan
    • 2
  • Saboohi Raza
    • 2
  • Yusuf Zafar
  • Rob W. Briddon
    • 1
  1. 1.National Institute for Biotechnology and Genetic EngineeringFaisalabadPakistan
  2. 2.Nuclear Institute for Agriculture (NIA)TandojamPakistan

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