A PCR-based method, with internal control, for the detection of Banana bunchy top virus in banana
- 310 Downloads
Banana bunchy top disease is a major constraint to banana production in most regions where this crop is grown. The disease is caused by Banana bunchy top virus (BBTV), a multicomponent, single-stranded DNA virus of the family Nanoviridae. We have designed primers to a conserved region of the master replication-associated protein that are useful for the polymerase chain reaction (PCR)-mediated detection of BBTV. In addition, primers to banana genomic sequence are used as an internal control, overcoming the uncertainty (owing to false-negatives) inherent in PCR diagnostics. Together these primer sets are a valuable tool in the effort to control BBTV, particularly in screening micropropagated banana plantlets for the absence of virus before release to farmers.
Index EntriesNanovirus diagnostics banana PCR banana bunchy top disease
Unable to display preview. Download preview PDF.
- 1.Soomro, M. H., Khalid, S., and Aslam, M. (1992) Outbreak of banana bunchy top virus in Sindh, Pakistan. FAO Plant Prot. Bull. 40/3, 95–100.Google Scholar
- 3.Vetten, H. J., Chu, P. W. G., Dale, J. L., et al. (2004) Nanoviridae. In: Virus Taxonomy, VIIIth Report of the ICTV (Fauquet, C. M., Mayo, M. A., Maniloff, J., Desselberger, U., and Ball, L. A., eds.), pp. 343–352. Elsevier/Academic Press, London.Google Scholar
- 7.Doyle, J. J. and Doyle, J. L. (1987) A rapid isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 19, 11–15.Google Scholar