Molecular Biotechnology

, Volume 30, Issue 2, pp 151–154 | Cite as

Optimal amounts of fluorescent dye improve expression microarray results in tumor specimens

  • Ali Naderi
  • Ahmed A. Ahmed
  • Yanzhong Wang
  • James D. Brenton
  • Carlos Caldas
Research

Abstract

Expression microarrays have great potential for clinical use but variability of the results represents a challenge for reliable practical application. The amount of fluorescent dye used in microarray experiments is a significant source of variability that has not been systematically studied. Here we demonstrate that the quantity of Cy3 dye affects microarray results performed on tumor specimens. Signal-to-noise ratios and coefficients of variation are significantly improved by increasing Cy3 to 150–180 pmol, but any further increase does not improve the data. In conclusion, optimal amounts of dye reduce variability and improve reliability of expression microarray experiments.

Index Entries

Expression microarray labeling 

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Copyright information

© Humana Press Inc. 2005

Authors and Affiliations

  • Ali Naderi
    • 1
  • Ahmed A. Ahmed
  • Yanzhong Wang
  • James D. Brenton
  • Carlos Caldas
  1. 1.Cancer Genomics Program, Department of OncologyUniversity of Cambridge, Hutchison/MRC Research CenterCambridgeUK

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