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Molecular Biotechnology

, Volume 30, Issue 2, pp 143–149 | Cite as

Expressed sequence tag analysis of Eimeria-stimulated intestinal intraepithelial lymphocytes in chickens

  • Wongi Min
  • Hyun S. Lillehoj
  • Christopher M. Ashwell
  • Curtis P. van Tassell
  • Rami A. Dalloul
  • Lakshmi K. Matukumalli
  • Jae Y. Han
  • Erik P. Lillehoj
Research

Abstract

Intraepithelial lymphocytes (IELs) play a critical role in protective immune response to intestinal pathogens such as Eimeria, the etiologic agent of avian coccidiosis. A list of genes expressed by intestinal IELs of Eimeria-infected chickens was compiled using the expressed sequence tag (EST) strategy. The 14,409 ESTs consisted of 1851 clusters and 7595 singletons, which revealed 9446 unique genes in the data set. Comparison of the sequence data with chicken DNA sequences in GenBank identified 125 novel clones. This EST library will provide a valuable resource for profiling global gene expression in normal and pathogen-infected chickens and identifying additional unique immune-related genes.

Index Entries

Chickens intestinal lymphocytes expressed sequence tag (EST) expression cDNA library Eimeria 

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Copyright information

© Humana Press Inc. 2005

Authors and Affiliations

  • Wongi Min
    • 1
    • 7
  • Hyun S. Lillehoj
    • 1
  • Christopher M. Ashwell
    • 2
  • Curtis P. van Tassell
    • 3
  • Rami A. Dalloul
    • 1
  • Lakshmi K. Matukumalli
    • 4
  • Jae Y. Han
    • 5
  • Erik P. Lillehoj
    • 6
  1. 1.Animal Parasitic Diseases Laboratory, Animal & Natural Resources InstituteUS Dept. of AgricultureBeltsvilleUSA
  2. 2.Growth Biology Laboratory, Animal & Natural Resources InstituteUS Dept. of AgricultureBeltsvilleUSA
  3. 3.Bovine Functional Genomics Laboratory, Animal & Natural Resources InstituteUS Dept. of AgricultureBeltsvilleUSA
  4. 4.Bioinformatics & Computational BiologyGeorge Mason UniversityFairfaxUSA
  5. 5.School of Agricultural BiotechnologySeoul National UniversitySeoulKorea
  6. 6.Department of Pharmaceutical Sciences, School of PharmacyUniversity of MarylandBaltimoreUSA
  7. 7.Department of Animal Science and TechnologySunchon National UniversitySuncheon, ChonnamKorea

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