Molecular Biotechnology

, Volume 26, Issue 2, pp 147–163 | Cite as

MALDI-TOF mass spectrometry

A versatile tool for high-performance DNA analysis
  • Christian Jurinke
  • Paul Oeth
  • Dirk van den Boom


Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) has developed during the past decade into a versatile tool for biopolymer analysis. The aim of this review is to summarize this development and outline the applications, which have been enabled for routine use in the field of nucleic acid analysis. These include the anlaysis of mutations, the resequencing of amplicons with a known reference sequence, and the quantitative analysis of gene expression and allelic frequencies in complex DNA mixtures.

Index Entries

MALDI-TOF SNP analysis genotyping quantitative MALDI-TOF gene-expression analysis resequencing SNP discovery DNA pooling 


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Copyright information

© Humana Press Inc 2004

Authors and Affiliations

  • Christian Jurinke
    • 1
  • Paul Oeth
  • Dirk van den Boom
  1. 1.Sequenom, Inc., Johns Hopkins CourtSan Diego

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