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Molecular Biotechnology

, Volume 26, Issue 1, pp 35–38 | Cite as

Cloning genes from a library using a clustering strategy and PCR

  • S. Díaz Prado
  • N. Tarrío
  • M. E. Cerdán
  • M. I. González SisoEmail author
Research Protocol

Abstract

A new polymerase chain reaction (PCR)-based method is described for the isolation of clones of interest from a library when only part of a sequence is available. In actuality, this occurs with many genomes that have been partially sequenced using a random strategy. The method presented here, discriminating clusters by PCR (DCbyPCR), is a nonradioactive and improved alternative to colony hybridization.

Index Entries

Cloning colon hybridization PCR 

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References

  1. 1.
    Feldman, H. (2000) Génolevures—a novel approach to “evolutionary genomics.” FEBS Lett. 487, 1–2.CrossRefGoogle Scholar
  2. 2.
    Wésolowski-Louvel, M., Tanguy-Rougeau, C., and Fukuhara, H. (1988) A nuclear gene required for the expression of the linear DNA-associated killer system in the yeast Kluyveromyces lactis. Yeast 4, 71–81.CrossRefGoogle Scholar
  3. 3.
    Miller, J. H. (1992) A Short Course in Bacterial Genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.Google Scholar
  4. 4.
    Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.Google Scholar

Copyright information

© Humana Press Inc 2004

Authors and Affiliations

  • S. Díaz Prado
    • 1
  • N. Tarrío
    • 1
  • M. E. Cerdán
    • 1
  • M. I. González Siso
    • 1
    Email author
  1. 1.Department of Molecular and Cell BiologyUniversity of A CoruñaCoruñaSpain

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