Molecular Biotechnology

, Volume 25, Issue 1, pp 53–61

Mimotopes of tumor-associated T-cell epitopes for cancer vaccines determined with combinatorial peptide libraries

  • Tumenjargal Sherev
  • Karl-Heinz Wiesmüller
  • Peter Walden
Protocol

Abstract

Cytotoxic T-cells are the most important effector cells in immune responses against tumors. The identification of tumor-associated epitopes for these cells, therefore, has become a key aspect of the development of cancer vaccines. Here, we describe a new approach to the determination of tumor-associated T-cell epitopes which employs combinatorial peptide libraries with singly defined sequence positions in a randomized context. The analysis of the responses of a T-cell clone to these libraries yields the amino acid constituents of the epitope which can be combined to obtain mimotopes that are suitable as vaccine antigens for the induction of tumor-specific responses.

Index Entries

Brefeldin-A combinatorial peptide library SEREX, serological analysis of tumor antigens by recombinant cDNA expression cloning TAA, tumor-associated antigen TATE, tumor-associated T-cell epitope 

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Copyright information

© Humana Press Inc 2003

Authors and Affiliations

  • Tumenjargal Sherev
    • 1
  • Karl-Heinz Wiesmüller
    • 2
  • Peter Walden
    • 1
  1. 1.Department of Dermatology and Allergy, Medical Faculty CharitéHumboldt UniversityBerlinGermany
  2. 2.EMC Microcollections GmbH and Institute of Organic ChemistryTübingenGermany

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