Mimotopes of tumor-associated T-cell epitopes for cancer vaccines determined with combinatorial peptide libraries
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Cytotoxic T-cells are the most important effector cells in immune responses against tumors. The identification of tumor-associated epitopes for these cells, therefore, has become a key aspect of the development of cancer vaccines. Here, we describe a new approach to the determination of tumor-associated T-cell epitopes which employs combinatorial peptide libraries with singly defined sequence positions in a randomized context. The analysis of the responses of a T-cell clone to these libraries yields the amino acid constituents of the epitope which can be combined to obtain mimotopes that are suitable as vaccine antigens for the induction of tumor-specific responses.
Index EntriesBrefeldin-A combinatorial peptide library SEREX, serological analysis of tumor antigens by recombinant cDNA expression cloning TAA, tumor-associated antigen TATE, tumor-associated T-cell epitope
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- 4.De Plaen, E., Lurquin, C., Brichard, V., et al. (1997) Cloning of genes coding for antigens recognized by cytolytic T lymphocytes. In: The Immunology Methods Manual. (Lefkovits, I., ed.), Academic Press Ltd., New York, pp. 692–718.Google Scholar
- 6.Rammensee, H. G., Bachmann, J. and Stevanovic, S. (1997) MHC Ligands and Peptide Motifs. Landes Bioscience, Georgetown, TX.Google Scholar
- 10.Furka, A. (1996) Chemical synthesis of peptide libraries. In: Combinatorial Peptide and Nonapeptide Libraries. (Jung, G. ed.), VCH Verlagsgesellschaft mbH, Weinheim, Germany.Google Scholar
- 22.Udaka, K., Wiesmüller, K. H., Kienle, S., et al. (1995) Tolerance to amino acid variations in peptides binding to the major histocompatibility complex class I protein H-2Kb. J. Biol. Chem. 270, 24,130–24,134.Google Scholar