Molecular Biotechnology

, Volume 23, Issue 1, pp 83–86 | Cite as

Universal primers for the PCR-mediated amplification of DNA 1: A satellite-like molecule associated with begomovirus-DNA β complexes

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Abstract

DNA 1 is a single-stranded DNA molecule of approximately 1370 nucleotides. It is associated with monopartite geminiviruses of the genus Begomovirus, which require a DNA β component for symptomatic infection. The DNA 1 molecule requires the helper begomovirus for movement in plants, but is capable of self-replication. We designed two abutting primer pairs (DNA101/DNA102 and UN101/UN102) to conserved sequences of DNA 1. This allowed polymerase chain reaction-mediated amplification of the full-length molecule from total nucleic acid extracts produced from various host plants from geographically distinct, worldwide locations. These primers are useful both as diagnostic probes and for producing full-length infectious clones for in planta studies.

Index Entries

DNA 1 begomovirus PCR oligonucleotide primers DNA β 

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© Humana Press Inc 2003

Authors and Affiliations

  1. 1.Department of Disease and Stress BiologyJohn Innes CentreNorwichUK

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