Magnetite-alginate beads for purification of some starch degrading enzymes
- 205 Downloads
Starch degrading enzymes, viz., β-amylase, glucoamylase, and pullulanase, were purified using magnetite-alginate beads. In each case, the enzyme activity was eluted by using 1.0 M maltose. β-Amylase (sweet potato), glucoamylase (Aspergillus niger), and pullulanase (Bacillus acidopullulyticus) from their crude preparations were purified 37-, 31-, and 49-fold with 86, 87, and 95% activity recovery, respectively.
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed single band in each case.
Index EntriesAffinity separation β-amylase glucoamylase pullulanase magnetite-alginate beads Aspergillus niger Bacillus acidopullulyticus
Unable to display preview. Download preview PDF.