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Molecular Biotechnology

, Volume 18, Issue 1, pp 57–61 | Cite as

Cell adhesion assays

Protocol

Abstract

Cell adhesion makes an important contribution to the maintenance of tissue structure, the promotion of cell migration, and the transduction of information about the cell microenvironment across the plasma membrane. An ability to quantitate adhesion has proven to be extremely valuable for those researchers studying the molecular mechanisms underlying these processes. This article will outline in detail two standard assays used for quantitating the adhesion of cells to an immobilized substrate. First, an attachment assay, which employs a colorimetric detection of bound cells, and second, a spreading assay, which employs phase contrast microscopy to measure the flattening of adherent cells.

Index Entries

Adhesion integrin spreading attachment extracellular matrix 

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References

  1. 1.
    Kueng, W., Silber, E., and Eppenberger, U. (1989) Quantification of cells cultured on 96-well plates. Anal. Biochem. 182, 16–19.PubMedCrossRefGoogle Scholar
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    Yamada, K. M. and Kennedy, D. W. (1984) Dualistic nature of adhesive protein function: fibronectin and its biologically active peptide fragments can autoinhibit fibronectin function. J. Cell Biol. 99, 29–36.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc 2001

Authors and Affiliations

  1. 1.School of Biological SciencesUniversity of ManchesterManchester

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