Molecular Biotechnology

, Volume 17, Issue 3, pp 225–238

Scale-up of suspension and anchorage-dependent animal cells


DOI: 10.1385/MB:17:3:225

Cite this article as:
Griffiths, B. Mol Biotechnol (2001) 17: 225. doi:10.1385/MB:17:3:225


Alternative culture processes for laboratory scale-up (to 20 L) are described for both suspension and anchorage-dependent cells. Systems range from simple multiple culture units such as the roller bottle, through stirred suspension and microcarrier unit bioreactors, to highly sophisticated perfusion culture capable of maintaining cells at densities of about 108/mL. Critical parameters in scale-up are discussed, and the advantages and disadvantages of each culture system are critically evaluated.

Index Entries

Mammalian cell culture suspension culture microcarrier culture culture scale-up anchorage-dependent cells animal cell bioreactors 

Copyright information

© Humana Press Inc. 2001

Authors and Affiliations

  1. 1.SC&PPorton, SalisburyUK

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