Sequence analysis and tissue specific expression of human HOXA7
A 3071-bp fragment containing the human HOXA7 gene was sequenced. It contained two exons, one intron, and two polyadenylation signals (AATAAA) at positions 1844 and 2923. The exon encoded 230 aa residues, while the hexapeptide, homeodomain, and C-terminal acidic domains were detected. When the total sequences were compared with those of murine Hoxa-7, the 5′ untranslated region (UTR), exon I, intron, exon II, and 3′ UTR exhibited 99, 92, 65, 85, and 72% homology, respectively. Through Northern analysis, about a 1.9-kb transcript was detected in the fetal kidney. Minor transcripts of 1.5 and 1.1-kb were also detected in the fetal liver as well as in the kidney. In the case of adult tissues, most of the tissues tested (lung, liver, skeletal muscle, kidney, pancreas, and placenta), except brain tissue, expressed a 5.3 kb transcript with various intensities. Our results here suggested that not only Abd-B type Hox genes, but the ones in paralogous groups I–VIII could generate multiple transcripts. The characterization of these tissue-specific and stage-specific alternative transcripts would help to understand the regulatory lunction of the HOXA7 gene during development, and possibly the pathology of human disease caused by Hox genes.
Index EntriesDevelopment expression genomic structure HOXA7 gene human
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- 4.Zelster, L., Desplan, C., and Heintz, N. (1996) Hoxb-13: a new Hox gene in a distant region of the HOXB cluster maintains collinearity, Development 122, 2475–2484.Google Scholar
- 14.Borrow, J., Sherman, A. M., Stanton, V. P., Jr., Becher, R., Collins, T., Williams, A. J., Dube, I., Katz, F., Kwong, Y. L., Morris, C., Ohyashiki, K., Toyama, K., Rowley, J., and Housma, D. E. (1996) The t(7;11)(p15;p15) translocation in acute myeloid leukaemia fuses the genes for nucleoporin NUP98 and class I homeoprotein HOXA9, Nat. Genet. 12, 159–167.PubMedCrossRefGoogle Scholar
- 16.Puschel, A. W., Balling, R., and Gruss, P. (1991) Separate elements cause restriction and specify boundries of Hox-1.1 expression, Derelopment 112, 279–287.Google Scholar
- 18.Shen, W. F., Largman, C., Lowney, P., Corral, J. C., Detmer, K., Hauser, C. A., Simonitch, T. A., Hack, F. M., and Lawrence, H. J. (1989) Lineage-restricted expression of homeobox-containing gene in human hematopoietic cell lines, Proc. Natl. Acad. Sci. USA 86, 8536–8540.PubMedCrossRefGoogle Scholar
- 23.Sham, M. H., Hunt, P., Nonchev, S., Papalopulu, N., Graham, A., Boncinelli, E., and Krumlauf, R. (1992) Analysis of murine Hox-2.7 gene: conserved alternative transcripts with differential distributions in the nervous system and the potential for shared regulatory regions, EMBO J. 11, 1825–1836.PubMedGoogle Scholar