Molecular Biotechnology

, Volume 12, Issue 1, pp 13–23

Virus crystallography

  • Elizabeth E. Fry
  • Jonathan Grimes
  • David I. Stuart
Review

Abstract

Virus crystallography can provide atomic resolution structures for intact isometric virus particles and components thereof. The methodology is illustrated by reference to a particularly complex example, the core of the bluetongue virus (700 Å).

Index Entries

X-ray diffraction virus structure crystal cryo electron microscopy 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Chiu, W., Burnett, R. M., and Garcea, R. L. eds (1997) Structural Biology of Viruses. Oxford University Press, New York.Google Scholar
  2. 2.
    Arnold E., et al. (1987) The structure determination of a common cold virus, human rhinovirus 14. Acta Cryst., A43, 346–361.Google Scholar
  3. 3.
    Luo M., Vriend G. Kamer G., and Rossmann M. G. (1989) Structure determination of Mengo virus. Acta Cryst., B45, 85–92.Google Scholar
  4. 4.
    Fry E., Acharya R., and Stuart D. (1993) Methods used in the structure determination of foot-and-mouth disease virus. Acta Cryst., A49, 45–55.Google Scholar
  5. 5.
    Rossmann M. G. (1989) The molecular replacement method. Acta Cryst., A46, 73–82.Google Scholar
  6. 6.
    Harrison S. C., Strong R. K., Schlesinger S., and Schlesinger M. J. (1992) Crystallisation of Sindbis virus and its nucleocapsid. J. Mol. Biol., 226, 277–280.PubMedCrossRefGoogle Scholar
  7. 7.
    Namba, K., Pattanyek, R. and Stubbs, G. (1989) Visualization of protein-nucleic acid interactions in a virus. Refined structure of tobacco mosaic virus at 2. 9Å resolution by X-ray fiber diffraction. J. Mol. Biol., 208, 307–325.PubMedCrossRefGoogle Scholar
  8. 8.
    Chen Z. et al. (1989) Protein-RNA interactions in an icosahedral virus at 3. 0Å resolution. Science, 245, 154–159.PubMedCrossRefGoogle Scholar
  9. 9.
    Böttcher, B., Wynne, S. A. and Crowther, R. A. (1997) Determination of the fold of the core proetin of hepatitis B virus by electron cryomicroscopy. Nature, 386, 88–91.PubMedCrossRefGoogle Scholar
  10. 10.
    Caspar D. L. D. and Klug A. (1962) Physical principles in the construction of regular viruses. Cold Sprint Harbor Symp. Quant. Biol., 27, 1–24.Google Scholar
  11. 11.
    Harlos K. (1992) Micro-bridges for sitting drop crystallizations. J. Appl. Cryst. 25, 536–538.CrossRefGoogle Scholar
  12. 12.
    Arndt U. (1990) Focussing optics for laboratory sources in X-ray crystallography, J. Appl. Cryst. 22, 53–60.Google Scholar
  13. 13.
    Grimes J. M., et al. (1998) The atomic structure of the bluetongue virus core. Nature, 395, 470–478.PubMedCrossRefGoogle Scholar
  14. 14.
    Stuart, D. I. and Jones, E. Y. (1993) Weissenberg data collection for macromolecular crystallography. Curr. Opin. Struct. Biol. 3, 737–740.CrossRefGoogle Scholar
  15. 15.
    S. Watasuki, pers. comm.Google Scholar
  16. 16.
    Sakabe, N. (1991) X-ray Diffraction Data Collection System for Modern Protein Crystallography with a Weissenberg Camera and an Imaging Plate using Synchrotron radiation. Nuclear Inst. Meth. Phys. Res [A], 303, 448–463.CrossRefGoogle Scholar
  17. 17.
    Hadfield, A. T. (1995) Structural studies on human rhinovirus 14 drug resitant compensation mutants. J. Mol. Biol., 253, 61–73.PubMedCrossRefGoogle Scholar
  18. 18.
    Rossmann M. G. and Erickson J. W. (1983) Oscillation photography of radiation-sensitive crystals using a synchrotron source. J. Appl. Cryst., 16, 629–636.CrossRefGoogle Scholar
  19. 19.
    Otwinowski, Z. O. and Minor, W. (1997) Processing of X-ray diffraction data collected in oscillation mode, in Methods in Enzymology, vol. 276 (Carter, C. W. and Sweet, R. M., eds.), Academic Press, pp. 307–326.Google Scholar
  20. 20.
    Lea S. and Stuart D. (1994) Deconvolution of fully overlapped reflections from crystals of foot and mouth disease virus O1 G67. Acta Cryst. D50, 160–167.Google Scholar
  21. 21.
    Tong L. and Rossmann M. G. (1990) The locked rotation function. Acta Cryst. A46, 783–792.Google Scholar
  22. 22.
    Brunger A. T., Kuriyan J. and Karplus M. (1987) Crystallographic R factor refinement by molecular dynamics. Science, 235, 458–460.PubMedCrossRefGoogle Scholar
  23. 23.
    Esnouf, unpublishedGoogle Scholar
  24. 24.
    Tsao J., Chapman M. S., Wu H., Agbandje M., Keller W., and Rossmann M. G. (1992) Structure determination of monoclinic canine parvovirus. Acta Cryst., B48, 75–88.Google Scholar
  25. 25.
    Kleygwegr, G. J., and Read, R. (1997) Not your average density. Structure, 5, 1557–1569.CrossRefGoogle Scholar
  26. 26.
    Fry, E., Logan, D., and Stuart, D. I. (1996) Virus crystallography, in Methods in Molecular Biology, Vol. 56, Crystallographic Methods and Protocols. (Jones, Mulloy, Sanderson, eds.), Humana Press, 319–363.Google Scholar
  27. 27.
    Rayment I. (1983) Molecular replacement method at low resolution: optimum strategy and intrinsic limitations as determined by calculations on icosahedral virus models. Acta Cryst. A39, 102–116.Google Scholar
  28. 28.
    Grimes, J. M., and Stuart, D. I. (1994) The use of the the free R-factor as a guide in paramter optimization for density modification, in Daresbury Study Weekend, (Bailey, Hubbard & Waller, eds.), pp. 67–76.Google Scholar
  29. 29.
    Wang B. C (1985) Resolution of phase ambiguity in macromolecular crystallography. Methods Enzymol. 115, 90–111.PubMedCrossRefGoogle Scholar
  30. 30.
    Morgunova, E., et al. (1994) The atomic structure of Carnation Mottle Virus capsid protein. FEBS letters, 338, 267–271.PubMedCrossRefGoogle Scholar
  31. 31.
    Jones T. A. (1985) Interactive computer graphics: FRODO, in Methods in Enzymology, vol. 115 (Wyckoff, H. W., Hirs, C. H. W., and Timasheff, S. N., eds.), Academic, Orlando, FL, pp. 157–171.Google Scholar
  32. 32.
    Jones T. A., et al. (1991) Improved methods for building protein models in electron density maps and the location of errors in these models. Acta Cryst. A47, 110–119.Google Scholar
  33. 33.
    Stuart & Fry, unpublished.Google Scholar
  34. 34.
    Grimes J. M., et al. (1997) An atomic model of the outer layer of the bluetongue virus core derived from X-ray crystallography and electron microscopy. Structure, 5, 885–893.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc 1999

Authors and Affiliations

  • Elizabeth E. Fry
    • 1
  • Jonathan Grimes
    • 1
  • David I. Stuart
    • 1
    • 2
  1. 1.Wellcome Trust Centre For Human GeneticsHeadingtonUK
  2. 2.Oxford Centre for Molecular SciencesOxfordUK

Personalised recommendations