Journal of Molecular Neuroscience

, Volume 26, Issue 1, pp 27–32 | Cite as

Oxidative stress activates transcription factor NF-κB-mediated protective signaling in primary rat neuronal cultures

  • Ella Kratsovnik
  • Yael Bromberg
  • Oded Sperling
  • Esther Zoref-Shani
Original Article

Abstract

Activation of transcription factor nuclear factor-κB (NF-κB) can result in enhanced de novo synthesis of both proteins that confer protection and those that cause death. The present study was undertaken to clarify in primary neuronal cultures the consequences of the oxidative stress-induced activation of NF-κB and mediation of death or survival signals. The neuronal cultures were exposed to chemical ischemia (iodoacetic acid), followed by reperfusion (I/R insult). This insult injured the neurons, as manifested in a 7- to 10-fold increase in LDH release, and decreased the cellular content of IκBα by 55–65 %, indicating NF-κB activation. The antioxidants LY231617, melatonin, and sodium salicylate and the antioxidant and inhibitor of NF-κB activation pyrrolidine dithiocarbamate, protected the neurons against the insult and prevented the decrease in cellular IκBα content. In contrast, inhibition of NF-κB translocation by SN50 in both uninsulated and insulted neuronal cultures resulted in a 2.9- and 2.4-fold increase in LDH release, respectively. The results indicate that the insult-induced oxidative stress activates transcription factor NF-κB associated with induction of protection and suggest that constitutive activation of NF-κB under physiological conditions acts to protect the neurons against physiological injury.

Index Entries

Antioxidants IκBα pyrrolidine dithiocarbamate (PDTC) reactive oxygen species (ROS) SN50 transcription factor nuclear factor-κB (NF-κB) 

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Copyright information

© Humana Press Inc 2005

Authors and Affiliations

  • Ella Kratsovnik
    • 1
  • Yael Bromberg
    • 1
  • Oded Sperling
    • 1
  • Esther Zoref-Shani
    • 1
  1. 1.Department of Clinical Biochemistry, Sackler Faculty of MedicineTel Aviv UniversityTel AvivIsrael

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