Individuality of Ag-selected and preimmune TCR repertoires
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We have combined flow cytometry and single-cell PCR to characterize the TCR BV repertoires selected by individual mice in a model CD8 response against a defined peptide/MHC complex (CW 3 170–179/Kd). Ourresults established that different mice select individually distinct yet structurally similar CW3-specific repertoires. Repertoire selection appears to be flexible depending on the immunizing cell dose. Using a single-donor, matched-pair-recipient adoptive transfer strategy, we demonstrated that the CW3-specific TCR repertoires of normal mice are already distinct at the preimmune level. We combine our data with computer simulations to test models for the composition of an Ag-specific preimmune repertoire and its selection during an immune response.
Key WordsTCR sequences Ag-selected repertoire Preimmune repertoire Single-cell PCR Adoptive transfer Computer simulations
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- 11.Kranz DM, Tjoa B: αβ T-cell receptor repertoires among cytotoxic and helper T lymphocytes: in M. Sitkovsky and P. Henkart (eds): Cytotoxic Cells. Boston, Birkhäuser, 1993, 49–57.Google Scholar
- 12.Casanova JL, Romero P, Widmann C, Kourilsky P, Maryanski JL: T cell receptor genes in a series of class 1 major histocompatibility complex-restricted cytotoxic T lymphocyte clones speqcific for a Plasmodium berghe inonapeptide: Implications for T cell allelic exclusion and antigen-specific repertoire. J Exp Med 1991;174: 1371–1383PubMedCrossRefGoogle Scholar
- 13.Boitel B, Ermonval M, Panina-Bordigmon P, Mariuzza RA, Lanzavecchia A, Acuto O: Preferential V beta gene usage and lack of junctional sequence conservation among human T cell receptors specific for a tetanus toxin-derived peptide: evidence for a dominant role of a germline-encoded V region in antigen/major histocompatibility complex recognition. J Exp Med 1992;175:765–777.PubMedCrossRefGoogle Scholar
- 17.MacDonald HR, Casanova JL, Maryanski JL, Cerottini JC: Oligoclonal expansion of major histocompatibility complex class 1-restricted cytolytic T lymphocytes during a primary immune response in vivo: direct monitoring by flow cytometry and polymerase chain reaction. J Exp Med 1993;177:1487–1492PubMedCrossRefGoogle Scholar
- 23.Maryanski JL, Casanova JL, Falk K, Gournier H, Jaulin C, Kourilsky P, Lemonnier FA, Luthy R, Rammensee HG, Rotzschke O, Servis C, Lopez JA: The diversity of antigen-specific TCR repertoires reflects the relative complexity of epitopes recognized. Hum Immunol 1997;54:117–128.PubMedCrossRefGoogle Scholar
- 24.Casanova JL, Martinon F, Gournier H, Barra C, Pannetier C, Regnault A, Kourilsky P, Cerottini JC, Maryanski JL: T cell receptor selection by and recognition of two class I major histocompatibility complex-restricted antigenic peptides that differ at a single position. J Exp Med 1993;177:811–820.PubMedCrossRefGoogle Scholar
- 25.Maryanski JL, Attuil V, Bucher P, Walker PR: A quantitative, single-cell PCR analysis of an antigen-specific TCR repertoire selected during an in vivo CD8 response: directevidence for a wide range of clonesizes with uniform tissue distribution. Mol Immunol 1999;36:745–753.PubMedCrossRefGoogle Scholar