Endocrine

, Volume 9, Issue 1, pp 65–69 | Cite as

Inhibitory effects of Serenoa repens on the kinetic of pig prostatic microsomal 5α-reductase activity

  • Marie-France Palin
  • Michel Faguy
  • Jean-Guy LeHoux
  • Ghislain Pelletier
Article

Abstract

The pathogenesis of benign prostatic hyperplasia is linked to the accumulation of dihydrotestosterone (DHT), the active form of testosterone (T), in prostatic tissue. We have defined characteristics of 5α-reductase enzyme which catalyzes the conversion of T into DHT in prostatic microsomes of growing pigs. Peaks for the 5α-reductase activity were found at pH 5.5 and 8.0, which indicates the presence of both type 1 and type 2 isozymes. Kinetic parameters of porcine 5α-reductase in the presence of Serenoa repens extracts revealed uncompetitive, noncompetitive, and mixed types of inhibitions. Our results show the inhibitory action of S. repens on prostate porcine microsomal 5α-reductase activity.

Key Words

Steroid 5α-reductase enzyme Serenoa repens benign prostatic hyperplasia (BPH) porcine pig swine testosterone (T) dihydrotestosterone (DHT) enzyme kinetics inhibitors prostate NADPH-dependent reductase 

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Copyright information

© Humana Press Inc. 1998

Authors and Affiliations

  • Marie-France Palin
    • 2
  • Michel Faguy
    • 2
  • Jean-Guy LeHoux
    • 1
  • Ghislain Pelletier
    • 2
  1. 1.Department of Biochemistry, Faculty of MedicineUniversity of SherbrookeCanada
  2. 2.Agriculture and Agri-Food CanadaDairy and Swine Research and Development CentreLennoxvilleCanada

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