Endocrine

, Volume 8, Issue 2, pp 123–134 | Cite as

The genomic structure and chromosomal location of the human TR2 orphan receptor, a member of the steroid receptor superfamily

Article

Abstract

Human TR2 orphan receptor, isolated from the testis and prostate, is a member of the steroid/thyroid hormone receptor superfamily. With the screening of a human genomic library and the combination of primer walking and PCR sequencing, we found that the entire TR2 orphan receptor gene coding region and 5′-untranslated region feature 13 introns and 14 exons, and that the consensus splice sequences (GT-AG) are present in all intron-exon boundaries. Within the region that codes for the DNA binding domain, TR2 orphan receptor gene has a distinct intron-exon junction. Whereas all other known steroid receptors have one splice site that separates their first and second zinc fingers in the DNA binding domain, TR2 orphan receptor has a rare splice site located in the middle of its first zinc finger. The identification of specific junction sequences for potential alternative splicing sites helps to explain the existence of multiple forms of TR2 orphan receptor cDNA (TR2-5, 7, 9, 11). The S1 nuclease protection assay for TR2 message revealed that there are multiple transcription initiations, and that the major cap site surrounded by an initiator-like sequence is located at the 104th nucleotide upstream from the translation start codon. Sequence analysis of a 2.7-kb DNA fragment upstream of the TR2 orphan receptor translation start codon unveiled several potential cis-acting elements, such as AP-1, HNF-5, GATA1 binding sites, and GC boxes. Using fluorescence in situ hybridization combined with a high-resolution G-banding technique, we found that the TR2 orphan receptor gene was mapped to human chromosome 12 at band q22, whereas the structurally closely related TR4 orphan receptor gene was mapped to human chromosome 3 at band q24.3.

Key Words

TR2 TR4 genomic structure chromosomal location transcription initiation 

Abbreviations

AR

androgen receptor

PR

progesterone receptor

RARβ

retinoic acid receptor β

CRBPII

cellular retinol binding protein II

DBD

DNA binding domain

LBD

ligand binding domain

RT-PCR

reverse transcriptase polymerase chain reaction

RXRβ

retinoid X receptor β

5′-RACE

5′-rapid amplification of cDNA end

GCTs

germ-cell tumors

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Copyright information

© Humana Press Inc 1998

Authors and Affiliations

  1. 1.University of Wisconsin Comprehensive Cancer CenterUniversity of WisconsinMadison
  2. 2.George Whipple Laboratory for Cancer Research, Department of Pathology, Urology, and BiochemistryUniversity of Rochester Medical CenterRochester

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