Cell Biochemistry and Biophysics

, Volume 41, Issue 3, pp 343–356

Visualization of the uptake of individual HDL particles in living cells via the scavenger receptor class B type I

  • Sebastian Rhode
  • Anton Breuer
  • Jan Hesse
  • Max Sonnleitner
  • Tamara A. Pagler
  • Marlon Doringer
  • Gerhard J. Schütz
  • Herbert Stangl
Original Article

Abstract

The scavenger receptor class B type I (SR-BI) plays an important role in mediating selective uptake of high-density lipoprotein (HDL)-derived cholesterol and cholesteryl ester in liver and steroidogenic tissues. The molecular mechanism by which this receptor mediates selective cholesteryl ester uptake remains still enigmatic. We applied ultrasensitive fluorescence microscopy to visualize the intracellular transport routes of HDL particles taken up via SR-BI in a Chinese hamster ovarian cell line. Although diffusion of the receptor bound particles on the cell surface is slow, internalization is accompanied by a dramatic increase in the mobility of the particles. HDL particles are endocytosed as clusters and actively transported to the perinuclear region of the cell. Costaining with organelle markers confirmed the involvement of an acidic compartment and the Golgi apparatus in the uptake process; finally, resecretion of the HDL particles was observed.

Index Entries

SR-BI HDL uptake selective cholesteryl ester uptake ultrasensitive microscopy retro-endocytosis chinese hamster ovarian cells 

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Copyright information

© Humana Press Inc 2004

Authors and Affiliations

  • Sebastian Rhode
    • 1
  • Anton Breuer
    • 1
  • Jan Hesse
    • 1
  • Max Sonnleitner
    • 2
  • Tamara A. Pagler
    • 3
  • Marlon Doringer
    • 3
  • Gerhard J. Schütz
    • 1
  • Herbert Stangl
    • 3
  1. 1.Institute for BiophysicsUniversity of LinzLinzAustria
  2. 2.Center for Biomedical NanotechnologyUpper Austrian Research GmbHLinzAustria
  3. 3.Institute for Medical ChemistryMedical University of ViennaViennaAustria

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