Cell Biochemistry and Biophysics

, Volume 41, Issue 2, pp 265–277

Messenger RNA decay in mammalian cells

The exonuclease perspective
  • David T. Fritz
  • Naomi Bergman
  • Walter J. Kilpatrick
  • Carol J. Wilusz
  • Jeffrey Wilusz
Review Article

Abstract

The majority of messenger RNA (mRNA) decay in mammalian cells appears to be the work of a series of RNA exoribonucleases. A set of multiple poly(A)-specific deadenylases has been identified, some, if not most, of which are likely to play a role in the key first step of mRNA turnover—the regulated shortening of the poly (A) tail. After deadenylation, the transcript likely gets degraded by either a 5′-to-3′ or a 3′-to-5′ exonucleolytic pathway. Interestigly, multiple exonucleases have been identified for both of these pathways that appear to form multicomponent complexes with diverse roles in cellular biology. Therefore these enzymes appear not only to be important components of the mRNA turnover machinery, but also may function in a networked fashion in the post-transcriptional control of gene expression.

Index Entries

mRNA decay exosome exonuclease deadenylase mRNA stability decapping AU-rich elements 

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Copyright information

© Humana Press Inc 2004

Authors and Affiliations

  • David T. Fritz
    • 1
  • Naomi Bergman
    • 2
  • Walter J. Kilpatrick
    • 2
  • Carol J. Wilusz
    • 2
  • Jeffrey Wilusz
    • 2
  1. 1.Department of Biochemistry and Molecular BiologyUMDNJ-New Jersey Medical SchoolNewark
  2. 2.Department of Microbiology, Immunology and PathologyColorado State UniversityFort Collins

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