Biological Trace Element Research

, Volume 93, Issue 1, pp 1–8

Manganese, copper, and zinc in cerebrospinal fluid from patients with multiple sclerosis

  • Torun M. Melø
  • Cecilie Larsen
  • Linda R. White
  • Jan Aasly
  • Torill E. Sjøbakk
  • Trond P. Flaten
  • Ursula Sonnewald
  • Tore Syversen
Article

DOI: 10.1385/BTER:93:1-3:1

Cite this article as:
Melø, T.M., Larsen, C., White, L.R. et al. Biol Trace Elem Res (2003) 93: 1. doi:10.1385/BTER:93:1-3:1

Abstract

The concentrations of manganese, copper, and zinc in cerebrospinal fluid (CSF) from patients with multiple sclerosis (MS) and patients with no known neurological disease (control group) were measured. Manganese and copper levels were determined by two different analytical methods: atomic absorption spectrometry (AAS) and high-resolution inductively coupled plasma-mass spectrometry (HR-ICP-MS), whereas zinc levels were determined by HR-ICP-MS only. Manganese levels (mean±SEM) were significantly decreased in the CSF of MS patients (1.07±0.13 µg/L, ICP-MS; 1.08±0.11 µg/L, AAS) compared to the levels in the control group (1.78±0.26 µg/L, ICP-MS; 1.51±0.17 µg/L, AAS). Copper levels were significantly elevated in the CSF of MS patients (10.90±1.11 µg/L; ICP-MS, 11.53±0.83 µg/L, AAS) compared to the levels in the control group (8.67±0.49 µg/L, ICP-MS; 9.10±0.62 µg/L, AAS). There were no significant differences between the CSF zinc levels of MS and control patients. The physiological basis for the differences in manganese and copper concentrations between MS patients and controls is unknown, but could be related to alterations in the manganese-containing enzyme glutamine synthetase and the copper-containing enzyme cytochrome oxidase.

Index Entries

Multiple sclerosis atomic absorption spectrometry HR-ICP-MS copper manganese zinc 

Copyright information

© Humana Press Inc. 2003

Authors and Affiliations

  • Torun M. Melø
    • 1
  • Cecilie Larsen
    • 1
  • Linda R. White
    • 2
    • 3
  • Jan Aasly
    • 3
  • Torill E. Sjøbakk
    • 3
  • Trond P. Flaten
    • 1
  • Ursula Sonnewald
    • 2
  • Tore Syversen
    • 2
  1. 1.Department of ChemistryNorwegian University of Science and TechnologyTrondheimNorway
  2. 2.Department of Clinical NeurosciencesNorwegian University of Science and TechnologyTrondheimNorway
  3. 3.Department of NeurologyRegional and University HospitalTrondheimNorway

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