Dietary silicon and arginine affect mineral element composition of rat femur and vertebra
Both arginine and silicon affect collagen formation and bone mineralization. Thus, an experiment was designed to determine if dietary arginine would alter the effect of dietary silicon on bone mineralization and vice versa. Male weanling Sprague-Dawley rats were assigned to groups of 12 in a 2×2 factorially arranged experiment. Supplemented to a ground corn/casein basal diet containing 2.3 µg Si/g and adequate arginine were silicon as sodium metasilicate at 0 or 35 µg/g diet and arginine at 0 or 5 mg/g diet. The rats were fed ad libitum deionized water and their respective diets for 8 wk. Body weight, liver weight/body weight ratio, and plasma silicon were decreased, and plasma alkaline phosphatase activity was increased by silicon deprivation. Silicon deprivation also decreased femoral calcium, copper, potassium, and zinc concentrations, but increased the femoral manganese concentration. Arginine supplementation decreased femoral molybdenum concentration but increased the femoral manganese concentration. Vertebral concentrations of phosphorus, sodium, potassium, copper, manganese, and zinc were decreased by silicon deprivation. Arginine supplementation increased vertebral concentrations of sodium, potassium, manganese, zinc, and iron. The arginine effects were more marked in the silicon-deprived animals, especially in the vertebra. Germanium concentrations of the femur and vertebra were affected by an interaction between silicon and arginine; the concentrations were decreased by silicon deprivation in those animals not fed supplemental arginine. The change in germanium is consistent with a previous finding by us suggesting that this element may be physiologically important, especially as related to bone DNA concentrations. The femoral and vertebral mineral findings support the contention that silicon has a physiological role in bone formation and that arginine intake can affect that role.
Index EntriesSilicon bone mineralization trace elements bone DNA
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- 3.E. M. Carlisle, Silicon, inHandbook of Nutritionally Essential Mineral Elements, B. L. O’Dell and R. A. Sunde, eds., Marcel Dekker, New York, pp. 603–618 (1997).Google Scholar
- 4.R. A. Wapnir, The absorption of arsenic, boron, silicon, aluminum, tin, iodine and fluorine: interactions with proteins and other nutrients, inProtein Nutrition and Mineral Absorption, R. A. Wapnir, ed., CRC, Boca Raton FL, pp. 277–308 (1990).Google Scholar
- 7.T. Gurney, Jr. and E. G. Gurney, DABA fluorescence assay for submicrogram amounts of DNA, inMethods in Molecular Biology, Vol. 2,Nucleic Acids, J. M. Walker, ed., Humana, Totowa, NJ, pp. 5–11 (1984).Google Scholar
- 10.SAS Institute, Inc.,SAS User’s Guide: Statistics Version, 5th ed., SAS Institute, Cary, NC (1985).Google Scholar
- 15.C. D. Seaborn and F. H. Nielsen, Dietary silicon effects acid and alkaline phosphatase and45calcium uptake in bone of rats,J. Trace Elements Exp. Med. 7, 11–18 (1994).Google Scholar
- 16.K. L. Watkins and L. L. Southern, Effect of dietary sodium zeolite A and graded levels of calcium on growth, plasma, and tibia characteristics of chicks,Poultry Sci. 70, 2295–2303 (1991).Google Scholar
- 17.R. M. Leach, Jr., B. S. Heinrichs, and J. Burdene, Broiler chicks fed low calcium diets. 1. Influence of zeolite on growth rate and parameters of bone metabolism,Poultry Sci. 69, 1539–1543 (1990).Google Scholar
- 18.J. Eisinger and D. Clairet, Effects of silicon, fluoride, etidronate and magnesium on bone mineral density: a retrospective study,Magnesium Res. 6, 247–249 (1993).Google Scholar
- 21.A. Schiano, F. Eisinger, P. Detolle, A. M. Laponche, B. Brisou, and J. Eisinger, Silicon, bone tissue and immunity (in French),Revue du Rhumatisme et des Maladies Osteo-Articulaires (Paris) 46, 483–486 (1979).Google Scholar