Zinc inhibits the mixed lymphocyte culture
- 86 Downloads
The mixed lymphocyte culture (MLC) is an established clinical method for bone marrow transplantation, as it serves as an in vitro model for allogenic reaction and transplantation. We previously showed that cytokine release into the supernatant is a more specific and sensitive parameter for cross-reactivity in the MLC than the common measurement of cell proliferation. Therefore we tried to find an inhibitor of the MLC in vitro with the least side effects in vivo, measuring interferon (IFN)-γ as one of the most important cytokines in posttransplant medicine. Earlier studies showed that zinc is an important trace element for immune function with both stimulatory and inhibitory effects on immune cells. We found that slightly elevated zinc concentrations (three to four times the physiological level), which do not decrease T-cell proliferation in vitro nor produce immunosuppressive effects in vivo, suppress alloreactivity in the mixed lymphocyte culture. In this report we analyzed the mechanism whereby zinc influences the MLC to possibly find a nontoxic way of immunosuppression.
Index EntriesMixed lymphocyte culture (MLC) mixed lymphocyte reaction (MLR) trace elements zinc
Unable to display preview. Download preview PDF.
- 3.S. G. Danzer and L. Rink, Cytokines in mixed lymphocyte culture as a prospective parameter for transplantation, Med. Klin. 91, 494–500 (1996).Google Scholar
- 21.J. C. Carel, R. D. Schreiber, L. Falqui, and P. E. Lacy, Transforming growth factor beta decreases the immuogenicity of rat islet xenografts (rat to mouse) and prevents rejection in association with treatment of the recipient with a monoclonal antibody to interferon gamma, Proc. Natl. Acad. Sci. (USA) 87, 1591–1595 (1990).CrossRefGoogle Scholar
- 29.A. E. Favier, The role of zinc in reproduction. Hormonal mechanisms, Biol. Trace Element Res. 32, 363–382 (1992).Google Scholar