Biological Trace Element Research

, Volume 115, Issue 1, pp 47–57 | Cite as

Influence of aqueous extract of Hibiscus sabdariffa L. petal on cadmium toxicity in rats

  • S. O. Asagba
  • M. A. Adaikpoh
  • H. Kadiri
  • F. O. Obi


The effects of chronic exposure to cadmium (Cd) on some selected biochemical parameters, as well as the possible protective role of aqueous extracts of Hibiscus sabdariffa L petal were studied in 12-wk-old male Wistar albino rats. Exposure to Cd caused a significant increase in plasma l-alanine aminotransferases (ALT) only but with a corresponding decrease in liver l-alanine and l-aspartate aminotransferases (L-ALT, L-AST) when compared to the Cd-free control. Total superoxide dismutase activity was decreased in the liver, testis, and prostate of Cd-exposed rats, whereas malondialdehyde (MDA) concentrations were increased relative to the Cd-free control. The metal significantly increased prostatic acid phosphatase activity in the prostate, but decreased the body weight gain of the rats and organ/body weight ratio for prostate and testis compared to the Cd-free control. Pretreatment of rats with aqueous extract of H. sabdariffa resulted in significantly less hepatotoxicity than with Cd alone as measured by plasma ALT and liver ALT and AST activities. The extract also protected the rats against Cd-induced liver, prostate, and testis lipoperoxidation as evidenced by significantly reduced MDA values in these organs, as well as reduced prostatic acid phosphatase activity in the prostate, when compared to the Cd-only exposed rats. Also, when compared to the organ/body weight ratios obtained from rats exposed to Cd alone the prostate and testis were protected by the extract as shown by enhanced prostate/body weight and testis/body weight ratios of Cd- and extract-treated rats. These data suggest that H. sabdarrifa L might be protective in Cd toxicity.

Index Entries

Cadmium Hibiscus sabdariffa hepatoprotection antioxidant enzymes phosphatase lipid peroxidation rats 


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  1. 1.
    WHO, Environment Health Criteria, 134, Cadmium, World Health Organization, Geneva (1992).Google Scholar
  2. 2.
    H. Hu, Heavy metal poisoning, in Harrison's Principles of Internal Medicine, 14th ed, A. S. Focicci, E. Braunwald, K. J. Issalbacher, et al., eds., McGraw-Hill, New York. pp. 2564–2569 (1998).Google Scholar
  3. 3.
    F. Williams, R. Robertson, and M. Poworth, Scottish Center for Infection and Environment Health: detail profile of 25 major organic and inorganic substances. SCEITH, Glasgow (1999).Google Scholar
  4. 4.
    S. J. Yin, C. L. Chem, J. Y. Sheu, W. C. Tseng, and T. H. Lin, Cadmium induced renal lipid peroxidation in rats and protection by selenium. J. Toxicol. Environ. Health. 57, 403–413 (1999).CrossRefGoogle Scholar
  5. 5.
    T. Yamano, S. D. Kosanke, and L. E. Rikans, Attenuation of cadmium induced liver injury in senescent male Fisher-344 rats. Role of metallothionein and glutathione, Toxicol. Appl. Pharmacol. 161, 225–230 (1999).PubMedCrossRefGoogle Scholar
  6. 6.
    P. B. Ryan, N. Huet, and D. L. Macintosh, Longitudinal investigation of exposure to arsenic, cadmium and lead in drinking water, Environ. Health Perspect. 108, 731–735 (2000).PubMedCrossRefGoogle Scholar
  7. 7.
    S. Telisma, P. Coi-Kovic, J. Jurasovic, A. Pizent, M. Gavella, and B. Roac, Semen quality and reproductive endocrine functions in relations to biomakers of lead, cadmium, zinc and copper in men, Environ. Health Perspect. 108, 45–53 (2000).CrossRefGoogle Scholar
  8. 8.
    J. A. Timbrell, Principles of Biochemical Toxicology, 2nd ed., Taylor & Francis, London (1991).Google Scholar
  9. 9.
    L. J. Goldwater and J. K. Clarkson, Cadmium, in Metallic Contaminants and Human Health, in D. Y. H. Lee, ed., Academic, New York, pp. 97–124 (1972).Google Scholar
  10. 10.
    H. Yoshioka, Y. Itai, and F. Mitsumori, 31P MNR study of acute toxic effects of cadmium chloride on rat liver, Magn. Reson. Med. 33(b), 795–800 (1995).PubMedCrossRefGoogle Scholar
  11. 11.
    D. Manca, A. C. Richard, B. Trottier, and G. Chevalier, Studies on lipid peroxidation in rats tissue following administration of low and moderate doses of cadmium chloride, Toxicology 67, 303–323 (1991).PubMedCrossRefGoogle Scholar
  12. 12.
    D. Bagchi, M. Bagchi, E. A. Hassoun, and S. J. Stohs, Cadmium induced excretion of urinary lipid metabolites, DNA damage, glutathione depletion and hepatic lipid peroxidation Sprague-Dawley rats. Biol. Trace Element Res. 52, 143–145 (1996).Google Scholar
  13. 13.
    S. Gupta, M. Arhar, J. R. Behari, and R. C. Srivastava, Cadmium mediated induction of cellular defense mechanism: a novel example for the development of adaptive response against a toxicant, Ind. Health 29, 1–9 (1991).PubMedCrossRefGoogle Scholar
  14. 14.
    S. Sarker, P. Yadav, R. Trivedi, A. K. Bansal, and D. Bhatnagar, Cadmium-induced lipid peroxidation and status of the antioxidant system in rat tissues, J. Trace Elements Med. Biol. 9(3), 144–149 (1995).Google Scholar
  15. 15.
    H. Horiguchi, M. Sato, N. Konno, and M. Fukushima, Long-term cadmium exposure induces anaemia in rats through hypoinduction of ertheoprotein in the kidneys, Arch. Toxicol. 71, 11–19 (1996).PubMedCrossRefGoogle Scholar
  16. 16.
    WHO, Evaluation of Certain Food Additives and Contaminants, Forty First Report of the Joint FAO-WHO Expert Committee on Food Additives, World Health Organisation, Geneva (1993).Google Scholar
  17. 17.
    FAO-WHO, Toxicological Evaluation of Certain Food Additives and contaminants, WHO Food Additive Series, World Health Organization, Rome (1986).Google Scholar
  18. 18.
    M. P. Waalkes, S. Rehn, A. O. Parantoni, and T. P. Coogan, Cadmium exposure in rats and tumours of the prostate, in Cadmium in the Human Environment: Toxicity and Carcinogenicity, G. F. Nordberg, R. F. M. Herber, and L. Alessio, eds., International Agency for Research on Cancer, Lyon, pp. 391–399 (1992).Google Scholar
  19. 19.
    V. Verougstrael, D. Lison, and P. Hots, Cadmium in lung and prostate cancer. a systematic review of recent epidemiological data, J. Toxicol. Environ. Health B: Crit. Rev. 6(3), 227–255 (2003).CrossRefGoogle Scholar
  20. 20.
    K. Robalds and P. Worsfold, Cadmium: toxicology and analysis. A review, Analysis 116, 549–568 (1991).Google Scholar
  21. 21.
    L. J. Casarett, and J. Doull, Casarett and Doull's Toxicology, Macmillan, New York (1986).Google Scholar
  22. 22.
    E. Kowalczyk, A. Kopff, P. Fijalkowski, et al. Effect of anthocyanins, on selected biochemical parameters in rats exposed to cadmium, Acta Biochem. Polon. 50(2), 543–548 (2003).Google Scholar
  23. 23.
    L. M. Perry, Medicinal Plants, of East and Southern Asia, MIT Press, Cambridge, MA (1980).Google Scholar
  24. 24.
    B. R. Christie, The Handbook of Plant Science in Agriculture, CRC, Boca Raton, FL (1984).Google Scholar
  25. 25.
    T. H. Tseng, E. S. Kao, C. Y. Chu, F. P. Chou, H. W. Lin Wu, and C. J. Wang, Protective effect of dried flower extract of Hibiscus sabdariffa L. against oxidative stress in rats primary hepatocytes, Food Chem. Toxicol. 35,(12), 1159–1164 (1997).PubMedCrossRefGoogle Scholar
  26. 26.
    D. W., Moss, Acid Phosphatase, in Methods of Enzymatic Analysis, 3rd ed., H. U. Bergmeyer, ed., Academic Press, Vol. 4, pp 92–106 (1984).Google Scholar
  27. 27.
    S. Reithman and S. Frankel, A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminases, Am. J. Clin. Pathol. 28, 56–63 (1957).Google Scholar
  28. 28.
    J. M. C. Gutteridge and S. Williams, Copper dependent hydroxyl radical damage to ascorbic acid, FEBS Lett. 137, 327–329 (1982).PubMedCrossRefGoogle Scholar
  29. 29.
    H. P. Misra and I. Fridovich, The role of superoxide ion in the autooxidation of epinephrine and a simple assay for superoxide dismutase, J. Biol. Chem. 247, 3170–3175 (1972).PubMedGoogle Scholar
  30. 30.
    S. O. Asagba, G. E. Eriyamremu, M. A. Adaikpoh, and A. Ezeoma, Levels of lipid peroxidation, superoxide dismutase and Na+/K+- ATPase in some tissues of rats exposed to a Nigerian-like diet and cadmium, Biol. Trace Element Res. 100(1), 075–086 (2004).CrossRefGoogle Scholar
  31. 31.
    E. Casalino, C. Stano, and C. Landriscina, Enzyme activity alteration by cadmium administration to rats: the possibility of iron involvement in lipid peroxidation, Arch. Biochem. Biophys. 346(2), 171–179 (1997).PubMedCrossRefGoogle Scholar
  32. 32.
    J. M. Sauer, M. P. Waalkes, S. B. Hooser, K. K. Kuester, C. A. McQueen, and I. G. Spies, Suppression of Kupffer cell function prevents cadmium-induced hepatocellular necrosis in male Sprague-Dawley rat, Toxicology 121, 155–164 (1997).PubMedCrossRefGoogle Scholar
  33. 33.
    J. Blasco and J. Puppo, Effects of heavy metals (Cu, Cd and Pb) on aspartate and alanine aminotransferases in Ruditapes philippinaram (mollusc: bivalvia), Comp. Biochem. Physiol. C: Pharmacol. Toxicol. Endocrinol. 122, 253–263 (1999).CrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • S. O. Asagba
    • 1
  • M. A. Adaikpoh
    • 2
  • H. Kadiri
    • 1
  • F. O. Obi
    • 2
  1. 1.Department of BiochemistryDelta State UniversityAbrakaNigeria
  2. 2.Department of BiochemistryUniversity of BeninBenin CityNigeria

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