Plasma values of oxidants and antioxidants in acute brain hemorrhage
- 91 Downloads
The levels of oxidants xanthine oxidase (XO), nitric oxide (NO), and malondialdehyde (MDA) and of the antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and glutathione reductase (GRD) were determined in plasma within 24 h after onset of hemorrhagic stroke in 17 patients (9 men and 8 women, aged 60.7±11.5 yr) and in 20 healthy controls (12 men and 8 women, aged 62.5±8.3 yr). Compared to controls, the plasma SOD and total superoxide scavenger activi ties (TSSA) were significantly lower and the NO levels were significantly higher among the stroke patients. XO showed a slight, nonsignificant increase in the patients, but the levels of MDA, NSSA, GRD, and GSH-Px did not show any significant differences between the two groups. The hemorrhage volume was negatively, correlated with the initial score of the Glasgow Coma Scale and a positive correlation with lethal outcome, but it did not correlate significantly with any of the measured parameters. The results suggest that free radicals might play a role in the development of brain injury following brain hemorrhage.
Index EntriesBrain hemorrhage lipid peroxidation reactive oxygen species oxidative brain injury antioxidant enzymes xanthine oxidase nitric oxide malondialdehyde
Unable to display preview. Download preview PDF.
- 9.J. M. Braughler, L. A. Duncan, and R. L. Chase, The involvement of iron in lipid peroxidation, J. Biol. Chem. 261, 10,282–10,289 (1986).Google Scholar
- 17.H. Aebi, Catalase, in Methods of Enzymatic Analysis, H. U. Bergmeyer, ed., Verlag Chemie, Weinheim, pp. 673–678 (1974).Google Scholar
- 20.S. Hashimato, A new spectrophotometric assay method of xanthine oxidase in crude tissue homogenate, Anal. Biochem. 62, 425–435 (1974).Google Scholar
- 25.M. B. Plotnikov, A. A. Lobanov, and V. V. Ivanov, Activation of lipid peroxidation in the brain in cerebral hemorrhage, Bull. Eksp. Biol. Med. 105(6), 677–678 (1988).Google Scholar
- 43.J. P. Broderick, Volume of intracerebral hemorrhage. A powerful and easy-to-use predictor of 30-day mortality, Stroke 247, 987–993 (1993).Google Scholar