Applied Biochemistry and Biotechnology

, Volume 127, Issue 2, pp 125–131 | Cite as

Comparison of bacterial and phage display peptide libraries in search of target-binding motif

  • Mojca LunderEmail author
  • Tomaž Bratkovič
  • Bojan Doljak
  • Samo Kreft
  • Uroš Urleb
  • Borut Štrukelj
  • Nadja Plazar
Original Research Articles


Genetic engineering allows modification of bacterial and bacteriophage genes, which code for surface proteins, enabling display of random peptides on the surface of these microbial vectors. Biologic peptide libraries thus formed are used for high-throughput screening of clones bearing peptides with high affinity for target proteins. There are reports of many successful affinity selections performed with phage display libraries and substantially fewer cases describing the use of bacterial display systems. In theory, bacterial display has some advantages over phage display, but the two systems have never been experimentally compared. We tested both techniques in selecting streptavidin-binding peptides from two commercially available libraries. Under similar conditions, selection of phage-displayed peptides to model protein streptavidin proved convincingly better.

Index Entries

Phage display library bacterial display library affinity selection ligand peptide streptavidin 


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Copyright information

© Humana Press Inc. 2005

Authors and Affiliations

  • Mojca Lunder
    • 1
    Email author
  • Tomaž Bratkovič
    • 1
    • 2
  • Bojan Doljak
    • 1
  • Samo Kreft
    • 1
  • Uroš Urleb
    • 1
    • 2
  • Borut Štrukelj
    • 1
    • 3
  • Nadja Plazar
    • 4
  1. 1.Department of Pharmaceutical Biology, Faculty of PharmacyUniversity of LjubljanaLjubljanaSlovenia
  2. 2.Lek Pharmaceutical CompanyLjubljanaSlovenia
  3. 3.Jožef Stefan InstituteLjubljanaSlovenia
  4. 4.College of Health Care of IzolaUniversity of PrimorskaIzolaSlovenia

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