Applied Biochemistry and Biotechnology

, Volume 98, Issue 1–9, pp 717–732 | Cite as

Ethanol production from olive oil extraction residue pretreated with hot water

  • Ignacio Ballesteros
  • José Miguel Oliva
  • Maria José Negro
  • Paloma Manzanares
  • Mercedes Ballesteros


The olive pulp fraction contained in the residue generated in olive oil extraction by a two-step centrifugation process can be upgraded by using the cellulose fraction to produce ethanol and recovering high value phenols (tyrosol and hydroxytyrosol). Olive pulp was pretreated in a laboratory scale stirred autoclave at different temperatures (150–250°C). Pretreatment was evaluated regarding cellulose recovery, enzymatic hydrolysis effectiveness ethanol production by a simultaneous saccharification and fermentation process (SSF), and phenols recovery in the filtrate. The pretreatment of olive pulp using water at temperatures between 200°C and 250°C enhanced enzymatic hydrolysis. Maximum ethanol production (11.9 g/L) was obtained after pretreating pulp at 210°C in a SSF fed-batch procedure. Maximum hydroxytyrosol recovery was obtained in the liquid fraction when pretreated at 230°C.

Index Entries

Olive oil extraction by-products pretreatment simultaneous saccharification and fermentation ethanol hydroxytyrosol 


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Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  • Ignacio Ballesteros
    • 1
  • José Miguel Oliva
    • 1
  • Maria José Negro
    • 1
  • Paloma Manzanares
    • 1
  • Mercedes Ballesteros
    • 1
  1. 1.Renewable Energies DepartmentCentro De Investigaciones Energéticas Medioambientales Y Technológicas, (CIEMAT)Spain

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