Applied Biochemistry and Biotechnology

, Volume 98, Issue 1–9, pp 641–654 | Cite as

Cellulase adsorption and an evaluation of enzyme recycle during hydrolysis of steam-exploded softwood residues

  • Yanpin Lu
  • Bin Yang
  • David Gregg
  • John N. Saddler
  • Shawn D. Mansfield
Article

Abstract

The sugar yield and enzyme adsorption profile obtained during the hydrolysis of SO2-catalyzed steam-exploded Douglas-fir and posttreated steamexploded Douglas-fir substrates were determined. After hot alkali peroxide posttreatment, the rates and yield of hydrolysis attained from the posttreated Douglas-fir were significantly higher, even at lower enzyme loadings, than those obtained with the corresponding steam-exploded Douglas-fir. The enzymatic adsorption profiles observed during hydrolysis of the two substrates were significantly different. Ultrafiltration was employed to recover enzyme in solution (supernatant) and reused in subsequent hydrolysis reactions with added, fresh substrate. These recycle findings suggested that the enzyme remained relatively active for three rounds of recycle. It is likely that enzyme recovery and reuse during the hydrolysis of posttreated softwood substrates could lead to reductions in the need for the addition of fresh enzyme during softwood-based bioconversion processes.

Index Entries

Cellulases Douglas-fir ultrafiltration adsorption enzyme recycle hydrolysis bioconversion 

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Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  • Yanpin Lu
    • 1
  • Bin Yang
    • 1
  • David Gregg
    • 1
  • John N. Saddler
    • 1
  • Shawn D. Mansfield
    • 1
  1. 1.Forest Products Biotechnology, Department of Wood ScienceUniversity of British ColumbiaMain MallCanada

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