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Applied Biochemistry and Biotechnology

, Volume 98, Issue 1–9, pp 311–318 | Cite as

A technique for mycelial development of ectomycorrhizal fungi on agar media

  • Álvaro Alberto De AraújoEmail author
  • Sevastianos Roussos
Article

Abstract

A technique was established to study ectomycorrhizal fungi on agar media. Petri dishes, 60 mm in diameter, containing 10 mL of culture medium covered with a cellophane disk were used for easy collection of the mycelium after growth. For analysis of fungal biomass production, a sterilized cellophane sheet was placed on the medium’s surface. Inoculation was achieved by placing a mycelial block onto the center of the cellophane sheet and then incubating at 25°C in the dark. Colony radial growth was measured and biomass dry wt was determined. Fresh mycelia were homogenized with 10 mL of acetate buffer (pH 5.5) for enzyme analysis. A crude extract was obtained by adding all culture medium to 90 mL of distilled water and homogenizing in a Potter. Reducing sugars, enzyme concentration, and pH were determined. Three fungal strains, Suillus collinitus, Pisosithus arrhizus, and Hebeloma cylindrosporum, were grown in different culture media (potato dextrose agar or Pintro’s medium). Parameters measured over time included glucose concentration, phosphatase activity, biomass, and pH.

Index Entries

Ectomycorrhizal fungi physiology apical growth Pisolithus Suillus Hebeloma biomass phosphatase agar media 

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Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  • Álvaro Alberto De Araújo
    • 1
    Email author
  • Sevastianos Roussos
    • 2
  1. 1.Recém-doutor/CNPq, Bioaroma Laboratory, Food Engineering SchoolUNICAMP, C.P. 6121Campinas, S.P.Brazil
  2. 2.IRD (anciennement ORSTOM)-Laboratoire de MicrobiologieUniversité de Provence, CESB/ESIL case 925. 163Marseille Cedex 9France

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