Applied Biochemistry and Biotechnology

, Volume 96, Issue 1–3, pp 303–311

Immobilization of lactate dehydrogenase on tetraethylorthosilicate-derived sol-gel films for application to lactate biosensor

  • Asha Chaubey
  • Manju Gerard
  • V. S. Singh
  • B. D. Malhotra
Article

Abstract

Tetraethylorthosilicate (TEOS)-derived sol-gel films were utilized for the immobilization of lactate dehydrogenase (LDH) by physical adsorption and sol-gel/LDH/sol-gel sandwich configuration. An attempt was made to ascertain the optimum pH and temperature for the immobilized LDH. It was shown that TEOS-derived sol-gel films containing physically adsorbed LDH exhibited linearity from 0.5 to 4 mM, whereas those containing LDH in sandwich configuration showed linearity from 0.5 to 3 mMl-lactate. These sol-gel films, immobilized with LDH, were found to be stable for about 4 weeks at 4–10°C.

Index Entries

Immobilization lactate dehydrogenase tetraethylorthosilicate (TEOS) sol-gel lactate biosensor physical adsorption sandwich configuration 

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Copyright information

© Humana Press Inc. 2001

Authors and Affiliations

  • Asha Chaubey
    • 1
    • 2
  • Manju Gerard
    • 1
  • V. S. Singh
    • 2
  • B. D. Malhotra
    • 1
  1. 1.Biomolecular Electronics and Conducting Polymer Research GroupNational Physical LaboratoryNew DelhiIndia
  2. 2.Department of BiochemistryL.L.R.M. Medical CollegeMeerutIndia

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