Immobilization of lactate dehydrogenase on tetraethylorthosilicate-derived sol-gel films for application to lactate biosensor
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Tetraethylorthosilicate (TEOS)-derived sol-gel films were utilized for the immobilization of lactate dehydrogenase (LDH) by physical adsorption and sol-gel/LDH/sol-gel sandwich configuration. An attempt was made to ascertain the optimum pH and temperature for the immobilized LDH. It was shown that TEOS-derived sol-gel films containing physically adsorbed LDH exhibited linearity from 0.5 to 4 mM, whereas those containing LDH in sandwich configuration showed linearity from 0.5 to 3 mMl-lactate. These sol-gel films, immobilized with LDH, were found to be stable for about 4 weeks at 4–10°C.
Index EntriesImmobilization lactate dehydrogenase tetraethylorthosilicate (TEOS) sol-gel lactate biosensor physical adsorption sandwich configuration
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- 1.Wolfbeis, O. S. (1991), Fiber Optic Chemical Sensors & Biosensors, vols, 1 & 2, CRC, Boca Raton, FL.Google Scholar
- 7.Xie, B., Harborn, U., Mecklenburg, M., and Danielsson, B. (1994), Clin. Chem. 40/12, 2282–2287.Google Scholar
- 10.Pandey, P. C. (1992), Bull. Electrochem. 8(5), 212–221.Google Scholar
- 11.Gun, J. and Lev, O. (1995), Anal. Chem. 336, 95–106.Google Scholar
- 17.Brinker, C. J. and Scherer, G. W. (1989), Sol-gel Science, Academic Press, NY.Google Scholar
- 19.Lev, O., Tsionksy, L., Rabinonovich, L., Glezor, V., Sampath, S., Pankrator, I., and Gun, J. (1995), Anal. Chem. 67, 22A-30A.Google Scholar
- 20.Dave, C., Dunn, B., Valentine, J. S., and Zink, J. I. (1994), Anal. Chem. 66, 1120A-1127A.Google Scholar