Applied Biochemistry and Biotechnology

, Volume 91, Issue 1–9, pp 459–467 | Cite as

Production of biosurfactant from a new and promising strain of Pseudomonas aeruginosa PA1

  • L. M. Santa Anna
  • G. V. Sebastian
  • N. PereiraJr.
  • T. L. M. Alves
  • E. P. Menezes
  • D. M. G. Freire


The Pseudomonas aeruginosa PA1 strain, isolated from the water of oil production in Sergipe, Northeast Brazil, wasevaluated as a potential rhamnolipid type of biosurfactant producer. The production of biosurfactants was investigated using different carbon sources (n-hexadecane, paraffin oil, glycerol, and babassu oil) and inoculum concentrations (0.0016–0.008 g/L) The best results were obtained with glycerol as the substrate and an initial cell concentration of 0.004 g/L. AC:N ratio of 22.8 led to the greatest production of rhamnolipids (1700 mg/L) and efficiency (1.18 g of rhamnolipid/g of dry wt).

Index Entries

Production of biosurfactants glycolipids rhamnolipids Pseudomonas aeruginosa surface tension 


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Copyright information

© Humana Press Inc 2001

Authors and Affiliations

  • L. M. Santa Anna
    • 1
  • G. V. Sebastian
    • 1
  • N. PereiraJr.
    • 2
  • T. L. M. Alves
    • 3
  • E. P. Menezes
    • 4
  • D. M. G. Freire
    • 5
  1. 1.Centro de Pesquisas da Petrobras (Petrobras Research Center-CENPES)Rio de JaneiroBrazil
  2. 2.Esco la de QuímicaUFRRio de JaneiroBrazil
  3. 3.PEQ/COPPE/UFRJRio de JaneiroBrazil
  4. 4.Fundacão Tropical André ToselloSão PauloBrazil
  5. 5.Faculdade de FarmáciaUFRJRio de JaneiroBrazil

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