Applied Biochemistry and Biotechnology

, Volume 89, Issue 2–3, pp 209–216 | Cite as

Sol-gel-derived prussian blue-silicate amperometric glucose biosensor

  • Subramanian Bharathi
  • Ovadia LevEmail author


A new type of inorganic biosensor is introduced. The sensor comprises glucose oxidase enzymes encapsulated in a sol-gel-derived Prussian blue-silicate hybrid network. Glucose is detected by the biocatalytic reduction of oxygen followed by catalytic reduction of hydrogen peroxide by the Prusian blue catalyst. The sol-gel silicate entails a rigid encapsulating matrix, the Prussian blue provides chemical catalysis and charge mediation from the reduction site to the supporting electrode, and the enzyme is responsible for the biocatalysis. The feasibility of a dual optical/electrochemical mode of analysis is also demonstrated.

Index Entries

Sol-gel biosensor Prussian blue modified electrodes 


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Copyright information

© Humana Press Inc. 2000

Authors and Affiliations

  1. 1.Fredy and Nadine Herrmann School of Applied SciencesThe Hebrew University of JerusalemJerusalemIsrael

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