Applied Biochemistry and Biotechnology

, Volume 82, Issue 2, pp 141–151 | Cite as

Xylitol production from hardwood hemicellulose hydrolysates by Pachysolen tannophilus, Debaryomyces hansenii, and Candida guilliermondii

  • Attilio Converti
  • Patrizia Perego
  • José Manuel Domínguez
Article

Abstract

Three different yeasts, Pachysolen tannophilus, Debaryomyces hansenii, and Candida guilliermondii, were evaluated to ferment xylose solutions prepared from hardwood hemicellulose hydrolysates, among which P. tannophilus proved to be the most promising microorganism. However, the presence of both lignin-derived compounds (LDC) and acetic acid rendered a poor fermentation. To enhance the fermentation kinetics, different treatments to purify the hydrolysates were studied, including overliming, charcoal adsorption for LDC removal, and evaporation for acetic acid and furfural stripping. Under the best operating conditions assayed, 39.5g/L of xylitol were achieved after 96 h of fermentation, which corresponds to a volumetric productivity of 0.41 g/L·h and a yield of product on consumed substrate of 0.63 g p /gS.

Index Entries

Xylitol hemicellulose hydrolysate Debaryomyces hansenii Candida guilliermondii Pachysolen tannophilus 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Pepper, T. and Olinger, P. M. (1988), Food Technol. 42, 98–106.Google Scholar
  2. 2.
    Hyvönen, L., Koivistoinen, P., and Voirol, F. (1982), in Advances in Food Research, vol. 28, Chichester, C. O., Mrak, E. M., and Stewart, G. F., eds., Academic, New York, pp. 373–403.Google Scholar
  3. 3.
    Perego, P., Converti, A., Palazzi, E., Del Borghi, M., and Ferraiolo, G. (1990), J. Ind. Microbiol. 6, 157–164.CrossRefGoogle Scholar
  4. 4.
    Heikkilä, H., Hyöky, G., Rahkila, L., Sarkki, M. L., and Viljava, T. (1991), International Patent WO 91/10740.Google Scholar
  5. 5.
    Domínguez, J. M., Gong, C. S., and Tsao, G. T. (1996), Appl. Biochem. Biotechnol. 57–58, 49–56.Google Scholar
  6. 6.
    Parajó, J. C., Domínguez, H., and Domínguez, J. M. (1997), Process Biochem. 32, 599–604.CrossRefGoogle Scholar
  7. 7.
    Chen, L. F. and Gong, C. S. (1985), J. Food Sci. 50, 226–228.CrossRefGoogle Scholar
  8. 8.
    Roberto, I. C., Felipe, M. G. A., Lacis, L. S., and da Silva, S. S. (1991), Biores. Technol. 36, 271–275.CrossRefGoogle Scholar
  9. 9.
    Parajó, J. C., Domínguez, H., and Domínguez, J. M. (1995), Bioproc. Eng. 13, 125–131.Google Scholar
  10. 10.
    Amartey, S. and Jeffries, T. (1996), World J. Microb. Biotechnol. 12, 281–283.CrossRefGoogle Scholar
  11. 11.
    Leonard, R. H. and Hajny, G. J. (1945), Ind. Eng. Chem. 37, 390–395.CrossRefGoogle Scholar
  12. 12.
    Tran, A. V. and Chambers, R. P. (1985), Biotechnol. Lett. 7, 841–846.CrossRefGoogle Scholar
  13. 13.
    Tran, A. V. and Chambers, R. P. (1986), Enzyme Microb. Biotechnol. 8, 439–445.CrossRefGoogle Scholar
  14. 14.
    Roberto, I. C., Lacis, L. S., Barbosa, M. F. S., and de Mancilha, I. M. (1991), Process Biochem. 26, 15–21.CrossRefGoogle Scholar
  15. 15.
    Roberto, I. C., de Souza, C. A., Felipe, M. G. A., Sato, S., and de Castro, H. F. (1994), Biotechnol. Lett. 16, 1211–1216.CrossRefGoogle Scholar
  16. 16.
    Du Preez, J. C. (1994), Enzyme Microb. Technol. 16, 944–956.CrossRefGoogle Scholar
  17. 17.
    Wilson, J. J., Deschatelets, L., and Nishikawa, N. (1989), Appl. Microb. Biotechnol. 31, 592–596.CrossRefGoogle Scholar
  18. 18.
    Delgenes, J., Moletta, R., and Navarro, J. M. (1996), Enzyme Microb. Technol. 19, 220–225.CrossRefGoogle Scholar
  19. 19.
    Parajó, J. C., Domínguez, H., and Domínguez, J. M. (1996), Biores. Technol. 57, 179–185.CrossRefGoogle Scholar
  20. 20.
    Parajó, J. C., Domínguez, H., and Domínguez, J. M. (1996), Bioproc. Eng. 16, 39–43.CrossRefGoogle Scholar
  21. 21.
    Parajó, J. C., Domínguez, H., and Domínguez, J. M. (1996), Biotechnol. Lett. 18, 593–598.CrossRefGoogle Scholar
  22. 22.
    Parajó, J. C., Domínguez, H., and Domínguez, J. M. (1997), Enzyme Microb. Technol. 21, 18–24.CrossRefGoogle Scholar
  23. 23.
    Domínguez, J. M., Cruz, J. M., Roca, E., Domínguez, H., and Parajó, J. C. (1999), Appl. Biochem. Biotechnol. 81(2), 119–130.CrossRefGoogle Scholar
  24. 24.
    Strickland, R. C. and Beck, M. J. (1984), Proc. VI Int. Symp. Alcohol Fuels Technol. 2, 220–226.Google Scholar
  25. 25.
    Felipe, M. G. A., Mancilha, I. M., Vitolo, M., Roberto, I. C., da Silva, S. S., and Rosa, S. A. M. (1993), Arq. Biol. Technol. 36(1), 103–114.Google Scholar
  26. 26.
    Roberto, I. C., Felipe, M. G. A., de Mancilha, I. M., Vitolo, M., Sato, S., and da Silva, S. S. (1995), Biores. Technol. 51, 255–257.CrossRefGoogle Scholar
  27. 27.
    Pfeifer, M. J., da Silva, S. S., Felipe, M. G. A., Roberto, Y. C., and de Mancilha, I. M. (1996), Appl. Biochem. Biotechnol. 57/58, 423–430.CrossRefGoogle Scholar
  28. 28.
    Felipe, M. G. A., Vitolo, M. and Mancilha, I. M. (1996), Acta Biotecnologica 16, 73–79.CrossRefGoogle Scholar
  29. 29.
    Felipe, M. G. A., Vitolo, M., de Mancilha, I. M., and da Silva, S. S. (1997), J. Ind. Microb. Biotechnol. 18, 251–254.CrossRefGoogle Scholar
  30. 30.
    Roberto, I. C., Sato, S., de Mancilha, I. M., and Taqueda, M. E. S. (1995), Biotechnol. Lett. 17, 1223–1228.CrossRefGoogle Scholar
  31. 31.
    Roberto, I. C., Sato, S., and de Mancilha, I. M. (1996), J. Ind. Microb. 16, 348–350.CrossRefGoogle Scholar
  32. 32.
    Roberto, I. C., da Silva, S. S., Felipe, M. G. A., de Mancilha, I. M., and Sato, S. (1996), Appl. Biochem. Biotechnol. 57–58, 339–347.Google Scholar
  33. 33.
    Felipe, M. G. A., Alves, L. A., da Silva, S. S., Roberto, I. C., de Mancilha, I. M., and Almeida e Silva, J. B. (1996), Biores. Technol. 56, 281–283.CrossRefGoogle Scholar
  34. 34.
    Watson, N. E., Prior, B. A., and Lategan, P. M. (1984), Enzyme Microb. Technol. 6, 451–456.CrossRefGoogle Scholar
  35. 35.
    Lindén, T. and Hahn-Hägerdal, H. (1989), Enzyme Microb. Technol. 11, 583–589.CrossRefGoogle Scholar
  36. 36.
    Perego, P., Converti, A., Zilli, M., and Del Borghi, M. (1994), Bioproc. Eng. 10, 35–41.CrossRefGoogle Scholar
  37. 37.
    Maiorella, B., Blanch, H. W., and Wilke, C. R. (1983), Biotechnol. Bioeng. 125, 103–121.CrossRefGoogle Scholar
  38. 38.
    Parekh, S. R., Parekh, R. S., and Wayman, M. (1987), Process Biochem. 22, 85–91.Google Scholar
  39. 39.
    Parajó, J. C., Domínguez, H., and Domínguez, J. M. (1998), Biores. Technol. 66, 25–40.CrossRefGoogle Scholar
  40. 40.
    Beck, M. J. and Strickland, R. C. (1984), Biomass 6, 101–110.CrossRefGoogle Scholar
  41. 41.
    Converti, A. and Del Borghi, M. (1996), Acta Biotechnologica 16, 133–144.CrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • Attilio Converti
    • 1
  • Patrizia Perego
    • 1
  • José Manuel Domínguez
    • 2
  1. 1.Department of Chemical and Process Engineering G.B. BoninoUniversity of GenoaGenoaItaly
  2. 2.Department of Chemical EngineeringUniversity of Vigo (Campus Ourense)OurenseSpain

Personalised recommendations