Applied Biochemistry and Biotechnology

, Volume 79, Issue 1–3, pp 867–876

The nature of lignin from steam explosion/enzymatic hydrolysis of softwood

Structural features and possible uses scientific note
  • Sergey M. Shevchenko
  • Rodger P. Beatson
  • John N. Saddler


Effective utilization of the lignin by-product is a prerequisite to the commercial viability of ethanol production from softwood wastes using a steam explosion (SE)/enzymatic hydrolysis (EH)/fermentation process. Changes in the chemical composition of Douglas fir wood on SO2-catalyzed SE followed by EH were assessed using conventional analytical methods and new halogen-probetechniques. A significant solubilization of hemicelluloses was observed in the SE stage, the severity of which affected subsequent fermentation of cellulose and sorption of enzymes. SE of softwood resulted in dramatic changes in the chemical structure of lignin in the residual material involving chemical reactions via the benzyl cation. This leads to a more condensed lignin with partly blocked α-reaction centres. Possible uses for this lignin are discussed.

Index entries

Lignin steam explosion enzymatic hydrolysis softwood 


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Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • Sergey M. Shevchenko
    • 1
  • Rodger P. Beatson
    • 1
  • John N. Saddler
    • 1
  1. 1.Forest Products Biotechnology, Department of Wood ScienceUniversity of British ColumbiaVancouverCanada

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