Laccase from Trametes versicolor

Stability at temperature and alkaline conditions and its effect on biobleaching of hardwood kraft pulp
  • Maria E. A. De Carvalho
  • M. C. Monteiro
  • G. L. Sant'AnnaJr.


The enzyme laccase was produced by the white-rot fungus Trametes versicolor in repeated batches cultures with immobilized mycelium. Two different culture conditions were used. Enzymes produced were evaluated regarding their stability a thigh temperatures (55°C and 65°C) and at alkaline conditions (pH 7.0 and pH 8.0) having in view the application of these enzymes in biobleaching of hardwood Kraft pulp.

Biobleaching experiments were divided in two parts, enzymatic prebleaching followed by chemical bleaching. In the enzymatic prebleaching the enzyme laccase was used at two conditions of pH and temperature, whereas the reaction time was fixed at 1h in all pretreatments. In the chemical bleaching the DEDED and DEpDED sequences were used.

The enzyme action was evaluated by Kappa number, viscosity, and brightness at the end of bleaching sequences. There were obtained values of Kappa numbers lower than control assays, viscosities compatible with industrial pulps, and brightness higher than controls, when pulps were pretreated for 1 h with laccase at pH 8.0 and 55°C.

Index Entries

Trametes versicolor laccase biobleaching kappa number reduction brightness increase 


  1. 1.
    Arbeloa, M., Leseleuc, J., Goma, G., and Pommier, J. C. (1992), Tappi J. 23 (3), 215–221.Google Scholar
  2. 2.
    Archibald, F. S. (1992), Holzforschung 46(4), 305–310.CrossRefGoogle Scholar
  3. 3.
    Bourbonnais, R. and Paice, M. G. (1992) Appl. Microbiol. Biotechnol. 36, 823–827.CrossRefGoogle Scholar
  4. 4.
    Bourbonnais, R. and Paice M. G. (1996), Tappi J. 79(6), 199–204.Google Scholar
  5. 5.
    de Carvalho, M. E. A., Monteiro, M. C., Bon, E. P. S., and Sant'Anna, G. L., Jr. (1988), Appl. Biochem. Biotechnol. 70–72, 955–966.Google Scholar
  6. 6.
    Fujita, K., Kondo, R., Sakai, K., Kashino, Y., Nishida, T., and Takahara, Y. (1993), Tappi J. 76(1), 81–84.Google Scholar
  7. 7.
    Graves, J. W., Joyce, T. W., and Jameel, H. (1993), Tappi J. 76(7), 153–158.Google Scholar
  8. 8.
    Hamilton, J., Senior, D. J., Rodriguez, A., Santiago, D., Szwec, J., and Ragauskas, A. J. (1996), Tappi J. 79(4), 231–234.Google Scholar
  9. 9.
    Monteiro, M. C. and de Carvalho, M. E. A. (1997), in Proceedings of the 30th Pulp and paper Annual Meeting. São Paulo, Nov. 3–7, pp. 75–89.Google Scholar
  10. 10.
    Jager, A., Croan, S., and Kirk, T. K. (1985), Appl. Environ. Microbiol. 50 (5), 1274–1278.PubMedGoogle Scholar
  11. 11.
    Johansson, T., and Nyman, P. O. (1993), Arch. Biochem. Biophy. 300(1), 49–56.CrossRefGoogle Scholar
  12. 12.
    Kantelinen, A., Hortling, B., Sundquist, J., Linko, M., and Viikari, L. (1993), Holzforschung 47(4), 318–324.CrossRefGoogle Scholar
  13. 13.
    Paice, M. G., Reid, L. D., Bourbonnais, R., Archibald, F. S., and Jurasek, L. (1993), Appl. Environ. Microbiol. 59(1), 260–265.PubMedGoogle Scholar
  14. 14.
    Reid, I. D. and Paice, M. G. (1994), FEMS Microbiol. Rev. 13, 369–376.CrossRefGoogle Scholar
  15. 15.
    Paice, I. D., Jurasek, L., Ho, C., Bourbonnais, R., and Archibald, F. (1989), Tappi J. 21(5), 217–221.Google Scholar
  16. 16.
    Monteiro, M. C. and de Carvalho, M. E. A. (1998), Appl. Biochem. Biotechnol. 70–72, 983–993.CrossRefGoogle Scholar
  17. 17.
    Szklarz, G. D., Antibus, R. K., Sinsabaugh, R. L., and Linkins, A. E. (1989), Mycologia 81(2), 234–240.CrossRefGoogle Scholar
  18. 18.
    Mandels, M., Andreotti, R. and Roche, C. (1976), Biotechnol. Bioeng. Symp. 6, 21–33.PubMedGoogle Scholar

Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • Maria E. A. De Carvalho
    • 1
    • 2
  • M. C. Monteiro
    • 3
  • G. L. Sant'AnnaJr.
    • 3
  1. 1.Departamento de BiotecnologiaFaculdade de Engenharia Química de LorenaLorenaBrazil
  2. 2.Instituto de QuímicaUniversidade Federal do Rio de JaneiroRJ
  3. 3.COPPEUniversidade Federal do Rio de JaneiroBrazil

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