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Applied Biochemistry and Biotechnology

, Volume 135, Issue 3, pp 181–192 | Cite as

Resveratrol-induced cell inhibition of growth and apoptosis in MCF7 human breast cancer cells are associated with modulation of phosphorylated akt and caspase-9

  • Yan Li
  • Junye Liu
  • Xinping Liu
  • Kefei Xing
  • Yun Wang
  • Fuyang Li
  • Libo Yao
Original Research Articles

Abstract

Resveratrol (trans-3,4N,-5-trihydroxystilbene), a phytoalexin present in grapes and red wine, is emerging as a natural compound with potential anticancer properties. Here we show that resveratrol affects the growth of human breast cancer cell lines MCF7, MDA-MB-231, SK-BR-3, and Bcap-37 in a dose-dependent manner and that MCF7 is the most sensitive among the four cell lines. MCF7 cells treated with resveratrol showed typical characteristics of apoptosis including the poly (ADP-ribose) polymerase cleavage, TdT-mediated dUTP nick end labeling-positive staining, and morphologic changes. Phosphorylation of the oncogene product Akt was significantly reduced followed by decreased phosphorylation and increased processing of pro-caspase-9 on resveratrol treatment. These results indicate that resveratrol seems to exert its growth-inhibitory/apoptotic effect on the breast cancer cell line MCF7 via the Akt-caspase-9 pathway.

Index Entries

Apoptosis resveratrol MCF7 Akt caspase-9 

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Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Yan Li
    • 1
    • 2
  • Junye Liu
    • 1
    • 2
  • Xinping Liu
    • 1
    • 2
  • Kefei Xing
    • 1
    • 2
  • Yun Wang
    • 3
  • Fuyang Li
    • 1
  • Libo Yao
    • 1
    • 2
  1. 1.The Department of Biochemistry and Molecular BiologyThe Fourth Military Medical UniversityXi'anChina
  2. 2.The State Key Laboratory of Cancer BiologyThe Fourth Military Medical UniversityXi'anChina
  3. 3.Xi'an Greenpharma Natural Medicine LabXi'anChina

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