Applied Biochemistry and Biotechnology

, Volume 134, Issue 3, pp 223–232 | Cite as

Lactic acid bacteria production from whey

  • María Elena Mondragón-Parada
  • Minerva Nájera-Martínez
  • Cleotilde Juárez-Ramírez
  • Juvencio Galíndez-Mayer
  • Nora Ruiz-Ordaz
  • Eliseo Cristiani-Urbina
Original Research Articles


The main purpose of this work was to isolate and characterize lactic acid bacteria (LAB) strains to be used for biomass production using a whey-based medium supplemented with an ammonium salt and with very low levels of yeast extract (0.25 g/L). Five strains of LAB were isolated from naturally soured milk after enrichment in whey-based medium. One bacterial isolate, designated MNM2, exhibited a remarkable capability to utilize whey lactose and give a high biomass yield on lactose. This strain was identified as Lactobacillus casei by its 16S rDNA sequence. A kinetic study of cell growth, lactose consumption, and titratable acidity production of this bacterial strain was performed in a bioreactor. The biomass yield on lactose, the percentage of lactose consumption, and the maximum increase in cell mass obtained in the bioreactor were 0.165 g of biomass/g of lactose, 100%, and 2.0 g/L, respectively, which were 1.44,1.11, and 2.35 times higher than those found in flask cultures. The results suggest that it is possible to produce LAB biomass from a whey-based medium supplemented with minimal amounts of yeast extract.

Index Entries

Ammonium salts biomass lactic acid bacteria whey yeast extract 


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Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • María Elena Mondragón-Parada
    • 1
  • Minerva Nájera-Martínez
    • 1
  • Cleotilde Juárez-Ramírez
    • 1
  • Juvencio Galíndez-Mayer
    • 1
  • Nora Ruiz-Ordaz
    • 1
  • Eliseo Cristiani-Urbina
    • 1
  1. 1.Departamento de Ingeniería Bioquímica, Escuele Naciona de Ciencias Biológicasdel I.P.N. Carpio y Plan de Ayala, “Centro Operativo Naranjo”MéxicoMéxico

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