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Applied Biochemistry and Biotechnology

, Volume 129, Issue 1–3, pp 256–264 | Cite as

RSM analysis of the effects of the oxygen transfer coefficient and inoculum size on the xylitol production by Candida guilliermondii

  • Mariana Peñuela Vásquez
  • Maurício Bezerra
  • De SouzaJr.
  • Nei PereiraJr.
Session 1B: Enzyme Catalysis and Engineering

Abstract

Biotechnology production of xylitol is an excellent alternative to the industrial chemical process for the production of this polyalcohol. In this work the behavior of Candida guilliermondii yeast was studied when crucial process variables were modified. The K La (between 18 and 40/h) and the initial cell mass (between 4 and 10 g) were considered as control variables. A response surface methodology was applied to the experimental design to study the resulting effect when the control variables were modified. A regression model was developed and used to determine an optimal value that was further validated experimentally. The optimal values determined for K La and X 0 were 32.85/h and 9.86 g, respectively, leading to maximum values for productivity (1.628 g/h) and xylitol yield (0.708 g/g).

Index Entries

Xylitol response surface methodology analysis xylose 

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Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Mariana Peñuela Vásquez
    • 1
  • Maurício Bezerra
    • 1
  • De SouzaJr.
    • 1
  • Nei PereiraJr.
    • 1
  1. 1.Departamento de Engenharia BioquímicaUniversidade Federal do Rio de Janeiro, Centro de TecnologiaRio de JaneiroBrasil

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