RSM analysis of the effects of the oxygen transfer coefficient and inoculum size on the xylitol production by Candida guilliermondii
- 75 Downloads
Biotechnology production of xylitol is an excellent alternative to the industrial chemical process for the production of this polyalcohol. In this work the behavior of Candida guilliermondii yeast was studied when crucial process variables were modified. The K La (between 18 and 40/h) and the initial cell mass (between 4 and 10 g) were considered as control variables. A response surface methodology was applied to the experimental design to study the resulting effect when the control variables were modified. A regression model was developed and used to determine an optimal value that was further validated experimentally. The optimal values determined for K La and X 0 were 32.85/h and 9.86 g, respectively, leading to maximum values for productivity (1.628 g/h) and xylitol yield (0.708 g/g).
Index EntriesXylitol response surface methodology analysis xylose
Unable to display preview. Download preview PDF.
- 1.Mussatto, I. S. and e Roberto, I. C. (2002), Biotecnl. Ciência e Desenvolvimento 28, 24–39.Google Scholar
- 6.Sampaio, F. C., De Faveri, D., Mantovani, H. C., Passos, F. L., Perego, P., and Converti, A. (2005), J. Food Eng., in press.Google Scholar
- 9.Du Preez, J. C. and Van Der Walt, J. P. (1983), Biotechnol. Lett. 6, 395–400.Google Scholar
- 10.Vásquez, M. P. (2004), M.Sc. Dissertation. School of Chemistry of the, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.Google Scholar
- 11.Moser, A. (1988) Bioprocess Technol—Kinetics and Reactors, Springer-Verlag, NY.Google Scholar
- 14.Montgomery, D. C. (2001) Desing Analysis Experiments. 5th Edition, Wiley, New York.Google Scholar
- 15.StatSoft, Inc. (2001). statistica (Data analysis software system), version 6, www. statsoft.com.Google Scholar