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Applied Biochemistry and Biotechnology

, Volume 129, Issue 1–3, pp 226–233 | Cite as

Evaluation of cell recycle on Thermomyces lanuginosus xylanase a production by Pichia pastoris GS 115

  • Verônica Ferreira
  • Patricia C. Nolasco
  • Aline M. Castro
  • Juliana N. C. Silva
  • Alexandre S. Santos
  • Mônica C. T. Damaso
  • Nei PereiraJr.
Session 1B: Enzyme Catalysis and Engineering

Abstract

This work aims to evaluate cell recycle of a recombinant strain of Pichia pastoris GS115 on the Xylanase A (XynA) production of Thermomyces lanuginosus IOC-4145 in submerged fermentation. Fed-batch processes were carried out with methanol feeding at each 12h and recycling cell at 24, 48, and 72 h. Additionally, the influence of the initial cell concentration was investigated. XynA production was not decreased with the recycling time, during four cell recycles, using an initial cell concentration of 2.5 g/L. The maximum activity was 14,050 U/L obtained in 24h of expression. However, when the initial cell concentration of 0.25 g/L was investigated, the enzymatic activity was reduced by 30 and 75% after the third and fourth cycles, respectively. Finally, it could be concluded that the initial cell concentration influenced the process performance and the interval of cell recycle affected enzymatic production.

Index Entries

Xylanase A Pichia pastoris cell reutilization heterologous expression 

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Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Verônica Ferreira
    • 1
  • Patricia C. Nolasco
    • 1
  • Aline M. Castro
    • 1
  • Juliana N. C. Silva
    • 1
  • Alexandre S. Santos
    • 1
  • Mônica C. T. Damaso
    • 2
  • Nei PereiraJr.
    • 1
  1. 1.Escola de Química-Universidade Federal do Rio de JaneiroRio de JaneiroBrazil
  2. 2.Embrapa Agroindústria de Alimentos-Laboratório de Processos FermentativosRio de JaneiroBrazil

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