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Applied Biochemistry and Biotechnology

, Volume 128, Issue 2, pp 117–129 | Cite as

Surface modification of neural probes with conducting polymer poly(hydroxymethylated-3,4-ethylenedioxythiophene) and its biocompatibility

  • Yinghong Xiao
  • David C. Martin
  • Xinyan Cui
  • Mahesh Shenai
Article

Abstract

A novel conducting polymer, poly(hydroxymethylated-3,4-ethylenedioxy-thiophene) (PEDOT-MeOH), was electrochemically deposited onto the electrodes of micromachined neural probes. Uniformly distributed film was obtained from aqueous solution when doped with polystyrenesulfonate. The surface morphology was rough and had good cellular adhesion. Impedance spectroscopy showed that the magnitude of coated electrode was lower than that of the bare gold over a range of frequencies from 100 to 105 Hz. Since the biocompatibility of the interface between the neural probes and brain tissue plays an important role when the probes are implanted in the central nervous system for long-term application, biomolecules were incorporated into the coating. Nonapeptide CDPGYIGSR was codeposited as the counterion in the conducting films. The surface morphology of the coating was fuzzy, providing many bioactive sites for interaction with neural cells. The magnitude of impedance was as low as 53 kω at the biologically relevant frequency of 1 kHz. An in vitro experiment demonstrated that the neuroblastoma cells grew preferentially on the PEDOT-MeOH/CDPGYIGSR-coated electrode sites and spread beyond the electrode area.

Index Entries

Conducting polymer surface modification micromachined neural probe biocompatibility 

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Copyright information

© Humana Press Inc 2006

Authors and Affiliations

  • Yinghong Xiao
    • 1
    • 2
  • David C. Martin
    • 2
    • 3
    • 4
  • Xinyan Cui
    • 5
  • Mahesh Shenai
    • 6
  1. 1.School of Chemical EngineeringNanjing University of Science and TechnologyNanjingChina
  2. 2.Department of Materials Science and EngineeringThe University of MichiganAnn Arbor
  3. 3.Department of Macromolecular Science and Engineering CenterThe University of MichiganAnn Arbor
  4. 4.Department of Biomedical EngineeringThe University of MichiganAnn Arbor
  5. 5.Department of BioengineeringUniversity of PittsburghPittsburgh
  6. 6.Center for Biologic NanotechnologyThe University of MichiganAnn Arbor

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