Applied Biochemistry and Biotechnology

, Volume 115, Issue 1–3, pp 1115–1126 | Cite as

Effects of sugar inhibition on cellulases and β-glucosidase during enzymatic hydrolysis of softwood substrates

  • Zhizhuang Xiao
  • Xiao Zhang
  • David J. Gregg
  • John N. Saddler
Session 6A Biomass Pretreatment and Hydrolysis

Abstract

A quantitative approach was taken to determine the inhibition effects of glucose and other sugar monomers during cellulase and β-Glucosidase hydrolysis of two types of cellulosic material: Avicel and acetic acid-pretreated softwood. The increased glucose content in the hydrolysate resulted in a dramatic increase in the degrees of inhibition on both β-Glucosidase and cellulase activities. Supplementation of mannose, xylose, and galactose during cellobiose hydrolysis did not show any inhibitory effects on β-Glucosidase activity. However, these sugars were shown to have significant inhibitory effects on cellulase activity during cellulose hydrolysis. Our study suggests that high-substrate consistency hydrolysis with supplementation of hemicellulose is likely to be a practical solution to minimizing end-product inhibition effects while producing hydrolysate with high glucose concentration.

Index Entries

β-Glucosidase cellulase degree of inhibition softwood glucose hydrolysate 

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Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • Zhizhuang Xiao
    • 1
  • Xiao Zhang
    • 1
  • David J. Gregg
    • 1
  • John N. Saddler
    • 1
  1. 1.Forest Products BiotechnologyUniversity of British ColumbiaVancouverCanada

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