Applied Biochemistry and Biotechnology

, Volume 115, Issue 1–3, pp 989–1002

Combined steam pretreatment and enzymatic hydrolysis of starch-free wheat fibers

  • Beatriz Palmarola-Adrados
  • Mats Galbe
  • Guido Zacchi
Session 6A Biomass Pretreatment and Hydrolysis

Abstract

Steam treatment of an industrial process stream, denoted starch-free wheat fiber, was investigated to improve the formation of monomeric sugars in subsequent enzymatic hydrolysis for further bioconversion into ethanol. The solid fraction in the process stream, derived from a combined starch and ethanol factory, was rich in arabinose (21.1%), xylose (30.1%), and glucose (18.6%), in the form of polysaccharides. Various conditions of steam pretreatment (170–220°C for 5–30 min) were evaluated, and their effect was assessed by enzymatic hydrolysis with 2 g of Celluclast + Ultraflo mixture/ 100 g of starch-free fiber (SFF) slurry at 5% dry matter (DM). The highest overall sugar yield for the combined steam pretreatment and enzymatic hydrolysis, 52g/100 g of DM of SFF, corresponding to 74% of the theoretical, was achieved with pretreatment at 190°C for 10 min followed by enzymatic hydrolysis.

Index Entries

Wheat fibers steam pretreatment enzymatic hydrolysis starch-free fiber microwave oven 

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Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • Beatriz Palmarola-Adrados
    • 1
  • Mats Galbe
    • 1
  • Guido Zacchi
    • 1
  1. 1.Department of Chemical Engineering 1Lund UniversityLundSweden

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