Applied Biochemistry and Biotechnology

, Volume 114, Issue 1–3, pp 713–721 | Cite as

High-productivity continuous biofilm reactor for butanol production

Effect of acetate, butyrate, and corn steep liquor on bioreactor performance
  • Nasib Qureshi
  • Patrick Karcher
  • Michael Cotta
  • Hans P. Blaschek
Session 3—Bioprocessing, Including Separations


Corn steep liquor (CSL), a byproduct of the corn wet-milling process, was used in an immobilized cell continuous biofilm reactor to replace the expensive P2 medium ingredients. The use of CSL resulted in the production of 6.29 g/L of total acetone-butanol-ethanol (ABE) as compared with 6.86 g/L in a control experiment. These studies were performed at a dilution rate of 0.32 h−1. The productivities in the control and CSL experiment were 2.19 and 2.01 g/(L·h), respectively. Although the use of CSL resulted in a 10% decrease in productivity, it is viewed that its application would be economical compared to P2 medium. Hence, CSL may be used to replace the P2 medium. It was also demonstrated that inclusion of butyrate into the feed was beneficial to the butanol fermentation. A control experiment produced 4.77 g/L of total ABE, and the experiment with supplemented sodium butyrate produced 5.70 g/L of total ABE. The butanol concentration increased from 3.14 to 4.04 g/L. Inclusion of acetate in the feed medium of the immobilized cell biofilm reactor was not found to be beneficial for the ABE fermentation, as reported for the batch ABE fermentation.

Index Entries

Immobilized cell biofilm reactor butanol corn steep liquor sodium butyrate Clostridium beijerinckii BA101 sodium acetate 


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Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • Nasib Qureshi
    • 1
  • Patrick Karcher
    • 2
  • Michael Cotta
    • 1
  • Hans P. Blaschek
    • 2
  1. 1.United States Department of AgricultureNational Center for Agricultural Utilization Research, Fermentation BiotechnologyPeoria
  2. 2.Biotechnology & Bioengineering Group, Department of Food Science & Human NutritionUniversity of IllinoisUrbana

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