Applied Biochemistry and Biotechnology

, Volume 114, Issue 1–3, pp 403–416 | Cite as

Production of ethanol from cellulosic biomass hydrolysates using genetically engineered saccharomyces yeast capable of cofermenting glucose and xylose

Session 2—Introduction to Microbial Catalysis and Engineering


Recent studies have proven ethanol to be the idael liquid fuel for transportation, and renewable ligno cellulosic materials to be the attractive feed stocks for ethanol fuel production by fermentation. The major fermentable sugars from hydrolysis of most cellulosic biomass are D-glucose and D-xylose. The naturally occurring Saccharomyces yeasts that are used by industry to produce ethanol from starches and cane sugar cannot metabolize xylose. Our group at Purdue University succeded in developing genetically engineered Saccharomyces yeasts capable of effectively cofermenting glucose and xylose to ethanol, which was accomplished by cloning three xylose-metabolizing genes into the yeast. In this study, we demonstrated that our stable recombinant Sacharomyces yeast, 424A (LNH-ST), which contains the cloned xylose-metabolizing genes stably integrated into the yeast chromosome in high copy numbers, can efficiently ferment glucose and xylose present in hydrolysates from different cellulosic biomass to ethanol.

Index Entries

Ethanol Saccharomyces yeasts hydrolysate corn stover corn fiber xylose glucose glycerol xylitol 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Chandrakant, P. and Bisaria, V. S. (1998), Crit. Rev. Biotechnol. 18, 259–331CrossRefGoogle Scholar
  2. 2.
    Bothast, R. J., Nichols, N. N., and Dien, B. S. (1999), Biotechnol. Prog. 15, 867–875.PubMedCrossRefGoogle Scholar
  3. 3.
    Ho, N. W. Y., Chen, Z., and Brainard, A. (1998), Appl. Environ. Microbiol. 64, 1852–1859.PubMedGoogle Scholar
  4. 4.
    Ho, N. W. Y. and Tsao, G. T. (1998), US patent application #08/148, 581; patent no. 5,789, 210.Google Scholar
  5. 5.
    Ho, N. W. Y. and Chen Z. D., (1997), PCT patent no. W097/42307.Google Scholar
  6. 6.
    Ho, N. W. Y., Chen, Z., Brainard, A. P., and Sedlak, M. (1999), in Advances in Biochemical Engineering/Biotechnology, vol. 65, Tsao, G. T., ed., Springer-Verlag, Berlin Heiderberg, Germany, pp. 163–192.Google Scholar
  7. 7.
    Ho, N. W. Y., Chen, Z., Brainard, A. P., and Sedlak, M. (2000), in ACS Symposium Series 767, Anstas, P. T., Heine, G. H., and Williamson, T. C., eds., American Chemical Society, Washington, DC, pp. 142–159.Google Scholar
  8. 8.
    Sedlak, M., Edenberg, H., and Ho, N. W. Y. (2003), Enzyme. Microb. Technol. 33, 19–28.CrossRefGoogle Scholar
  9. 9.
    Toon, S. T., Philippidis, G. P., Ho, N. W. Y., Chen, Z., Brainard, A., Lumpkin, R. E., and Riley, C. J. (1997), Appl. Biochem. Biotechnol. 63/65, 243–255.CrossRefGoogle Scholar
  10. 10.
    Moniruzzaman, M., Dien, B. S., Skory, C. D., Chen, Z. D., Hespell, R. B., Ho, N. W. Y., Dale, B. E., and Bothast, R. J. (1997), World J. Microbiol. Biotech. 13, 341–346.CrossRefGoogle Scholar
  11. 11.
    Johansson, B., Christensson, C., Hobley, T., and Hahn-Hagerdal, B. (2001), Appl. Environ. Microbiol. 67, 4249–4255.PubMedCrossRefGoogle Scholar
  12. 12.
    Zadivar, J., Borges, A., Johansson, B., Smits, P. H., Villas-Boas, S. G., Nielsen, J., and Olsson, L. (2002), Appl. Microbiol. Biotechnol. 59, 436–442.CrossRefGoogle Scholar
  13. 13.
    Martin, C., Gable, M., Walbom, C. F., Hahn-Hagerdal, B., and Jonsson, L. J. (2002), Enzyme Microb. Technol. 31, 274–282.CrossRefGoogle Scholar
  14. 14.
    Martin, C., Gable, M., Nilvebrant, N.-O., and Jonsson, L. J. (2002), Appl. Biochem. Biotechnol. 98–100, 699–716.PubMedCrossRefGoogle Scholar
  15. 15.
    Eliasson, A., Christensson, C., Wahlbom, C. F., and Hahn-Hagerdal, B. (2000), Appl. Environ. Microbiol. 66, 3381–3386.PubMedCrossRefGoogle Scholar
  16. 16.
    Toivari, M. H., Aristidou, A., Rhuohonen, L., and Penttila, M. (2001), Metab. Eng. 3, 236–249.PubMedCrossRefGoogle Scholar
  17. 17.
    Jin, Y-S., Ni, H., Laplaza, J. M., and Jeffries, T. M. (2003), Appl. Environ. Microbiol. 69, 495–503.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  1. 1.Laboratory of Renewable Resources Engineering (LORRE)Purde UniversityWest Lafayette

Personalised recommendations