Applied Biochemistry and Biotechnology

, Volume 113, Issue 1–3, pp 307–319 | Cite as

Studies on immobilizd lipase in hydrophobic sol-gel

  • Cleide M. F. Soares
  • Onelia A. dos Santos
  • Heizir F. de Castro
  • Flavio F. de Moraes
  • Gisella M. ZaninEmail author


The hydrolysis of tetraethoxysilane using the sol-gel process was used to produce silica matrices, and these were tested for the immobilization of lipase from Candida rugosa by three methods: physical adsorption, covalent binding, and gel entrapment in the presence and absence of polyethylene glycol (PEG-1450). The silica matrices and their derivatives were characterized regarding particle size distribution, specific surface area, pore size distribution (Brunauer, Emmett, and Teller [B.E.T.] method), yield of grafting (thermogravimetric analyzer [TGA]), and chemical composition (Fourier transform infrared). Immobilization yields based on recovered lipase activity varied from 3.0 to 32.0%, and the highest efficiency was attained when lipase was encapsulated in the presence of PEG.

Index Entries

Silica matrices immobilization lipase additive sol-gel 


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Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • Cleide M. F. Soares
    • 1
  • Onelia A. dos Santos
    • 1
  • Heizir F. de Castro
    • 2
  • Flavio F. de Moraes
    • 1
  • Gisella M. Zanin
    • 1
    Email author
  1. 1.Department of Chemical EngineeringState University of MaringaMaringa-PRBrazil
  2. 2.Department of Chemical EngineeringFaculdade de Engenharia Quimica de LorenaLorena-SPBrazil

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