Applied Biochemistry and Biotechnology

, Volume 110, Issue 1, pp 53–60 | Cite as

Calcium alginate gel as encapsulation matrix for coimmobilized enzyme systems

  • A. BlandinoEmail author
  • M. Macías
  • D. Cantero


Encapsulation within calcium alginate gel capsules was used to produce acoimmobilized enzyme system. Glucose oxidase (GOD) and catalase (CAT) were chosen as model enzymes. The same values of V max and K mapp for the GOD encapsulated system and for the GOD-CAT coencapsulated system were calculated. When gel beads and capsules were compared, the same catalyst deactivation sequence for the two enzymes was observed. However, when capsules were employed as immobilization support, GOD efficiencies were higher than for the gel beads. These results were explained in terms of the structure of the capsules.

Index Entries

Calcium alginate gel capsules gel beads coimmobilization glucose oxidase catalase 


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Copyright information

© Humana Press Inc. 2003

Authors and Affiliations

  1. 1.Biological and Enzymatic Reactors Research Group, Department of Chemical Engineering, Food Technology and Environmental Technologies, Faculty of SciencesUniversity of Cádiz (UCA)Puerto Real (Cádiz)Spain

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