Calcium alginate gel as encapsulation matrix for coimmobilized enzyme systems
- 269 Downloads
Encapsulation within calcium alginate gel capsules was used to produce acoimmobilized enzyme system. Glucose oxidase (GOD) and catalase (CAT) were chosen as model enzymes. The same values of Vmax and Kmapp for the GOD encapsulated system and for the GOD-CAT coencapsulated system were calculated. When gel beads and capsules were compared, the same catalyst deactivation sequence for the two enzymes was observed. However, when capsules were employed as immobilization support, GOD efficiencies were higher than for the gel beads. These results were explained in terms of the structure of the capsules.
Index EntriesCalcium alginate gel capsules gel beads coimmobilization glucose oxidase catalase
Unable to display preview. Download preview PDF.
- 2.Chang, T. M. S., McIntosh, F. C., and Mason, F. G. (1966), Can. J. Physiol. Pharmacol. 44, 115.Google Scholar
- 3.Skjak-Braek, G. and Martinsen, A. (1991), in Seaweed Resources in Europe: Uses and Potential, Guiry, M. D. and Blunden, G., eds., CRC Press, New York, p. 219.Google Scholar
- 4.Christenson, L., Dionne, K., and Lysaught, M. (1993), in Fundamentals of Animal Cell Encapsulation, Goosen, F. A., ed., CRC Press, New York, pp. 7–41.Google Scholar
- 6.Linker, A. and Jones, R. S. (1966), J. Biol. Chem. 241, 3845–3851.Google Scholar
- 7.Haug, A. (1959), Acta Chem. Scand. 13, 601–603.Google Scholar
- 12.Romero, L. E. and Cantero, D. (1998), in Stability and Stabilization of Biocatalyst, Ballesteros, A., Plou, F. J., Iborra, J. L., and Halling, P. J., eds. Elsevier, New York, pp. 107–112.Google Scholar
- 13.Romero, L. E., Macías, M., and Cantero, D. (1998), Ingeniería Química 10, 189–193.Google Scholar
- 16.Boltz, D. F. and Howell, J. A. (1987), Colorimetric Determination of Nonmetals, John Wiley & Sons, New York.Google Scholar