Applied Biochemistry and Biotechnology

, Volume 105, Issue 1–3, pp 141–153 | Cite as

Effect of lignocellulosic degradation compounds from steam explosion pretreatment on ethanol fermentation by thermotolerant yeast Kluyveromyces marxianus

  • Jose Miguel Oliva
  • Felicia Sáez
  • Ignacio Ballesteros
  • Alberto González
  • Maria José Negro
  • Paloma Manzanares
  • Mercedes Ballesteros


The filtrate from steam-pretreated poplar was analyzed to identify degradation compounds. The effect of selected compounds on growth and ethanolic fermentation of the thermotolerant yeast strain Kluyveromyces marxianus CECT 10875 was tested. Several fermentations on glucose medium, containing individual inhibitory compounds found in the hydrolysate, were carried out. The degree of inhibition on yeast strain growth and ethanolic fermentation was determined. At concentrations found in the prehy-drolysate, none of the individual compounds significantly affected the fermentation. For all tested compounds, growth was inhibited to a lesser extent than ethanol production. Lower concentrations of catechol (0.96 g/L) and 4-hydroxybenzaldehyde (1.02 g/L) were required to produce the 50% reduction in cell mass in comparison to other tested compounds.

Index Entries

Ethanol production Kluyveromyces marxianus poplar biomass inhibitors fermentation 


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Copyright information

© Humana Press Inc. 2003

Authors and Affiliations

  • Jose Miguel Oliva
    • 1
  • Felicia Sáez
    • 1
  • Ignacio Ballesteros
    • 1
  • Alberto González
    • 1
  • Maria José Negro
    • 1
  • Paloma Manzanares
    • 1
  • Mercedes Ballesteros
    • 1
  1. 1.CIEMAT-Departmento de Energías RenovablesMadridSpain

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