Applied Biochemistry and Biotechnology

, Volume 102, Issue 1–6, pp 141–153

A biotechnological process involving filamentous fungi to produce natural crystalline vanillin from maize bran

  • Laurence Lesage-Meessen
  • Anne Lomascolo
  • Estelle Bonnin
  • Jean-Francois Thibault
  • Alain Buleon
  • Marc Roller
  • Michele Asther
  • Eric Record
  • Benoit Colonna Ceccaldi
  • Marcel Asther
Article

DOI: 10.1385/ABAB:102-103:1-6:141

Cite this article as:
Lesage-Meessen, L., Lomascolo, A., Bonnin, E. et al. Appl Biochem Biotechnol (2002) 102: 141. doi:10.1385/ABAB:102-103:1-6:141

Abstract

A new process involving the filamentous fungi Aspergillus niger and Pycnoporus cinnabarinus has been designed for the release of ferulic acid by enzymic degradation of a cheap and natural agricultural byproduct (autoclaved maize bran) and its biotransformation into vanillic acid and/or vanillin with a limited number of steps. On the one hand, the potentialities of A. niger I-1472 to produce high levels of polysaccharide-degrading enzymes including feruloyl esterases and to transform ferulic acid into vanillic acid were successfully combined for the release of free ferulic acid from autoclaved maize bran. Then vanillic acid was recovered and efficiently transformed into vanillin by P. cinnabarinus MUCL 39533, since 767 mg/L of biotechnologic vanillin could be produced in the presence of cellobiose and XAD-2 resin. On the other hand, 3-d-old high-density cultures of P. cinnabarinus MUCL39533 could be fed with the autoclaved fraction of maize bran as a ferulic acid source and a. niger I-1472 culture filtrate as an extracellular enzyme source. Under these conditions, P. cinnabarinus MUCL39533 was shown to directly biotransform free ferulic acid released from the autoclaved maize bran by A. niger I-1472 enzymes into 584 mg/L of vanillin. These processes, involving physical, enzymic, and fungal treatments, permitted us to produce crystallin vanillin from autoclaved maize bran without any purification step.

Index Entries

Aroma maize bran enzyme ferulic acid vanillic acid vanillin Aspergillus niger Pycnoporus cinnabarinus 

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Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  • Laurence Lesage-Meessen
    • 1
  • Anne Lomascolo
    • 1
  • Estelle Bonnin
    • 2
  • Jean-Francois Thibault
    • 2
  • Alain Buleon
    • 3
  • Marc Roller
    • 4
  • Michele Asther
    • 1
  • Eric Record
    • 1
  • Benoit Colonna Ceccaldi
    • 5
  • Marcel Asther
    • 1
  1. 1.Unité de Biotechnologie des Champignons Filamenteux de I’INRAIFR de Biotechnologie Agro-Industrielle de MarseilleMarseille Cedex 09France
  2. 2.Unité de Recherches sur les Polysaccharidesleurs Organisations et InteractionsFrance
  3. 3.Unité de Physico-Chimie des Macromolécules INRANantes Cedex 03France
  4. 4.Agro-IndustrieRecherche et Développement, Route de BazancourtPomacleFrance
  5. 5.Laboratoire de TechnologieCentre de Recherches Pernod-RicardCréteil CedexFrance

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