Applied Biochemistry and Biotechnology

, Volume 101, Issue 3, pp 239–249 | Cite as

pH-sensitive chitosan films for baker’s yeast immobilization

  • H. Nursevin Öztop
  • Dursun Saraydin
  • Şenay Cetinus


Dried baker’s yeast cells were immobilized on a chitosan film, which is a natural polymer. Prepared chitosan films were treated with glutaraldehyde to facilitate the immobilization of the cells. The effects of the amount of glutaraldehyde, incubation time, pH, and temperature on immobilization were investigated. The amount of glutaraldehyde was chosen to be 0.01% (weight). The highest amount of yeast immobilization was obtained with 5 h incubation. It was determined that optimum temperature for immobilization is 25°C, and the optimum pH for immobilization is 6. Immobilized cells were allowed to stand for 3 d in distilled water and buffer solution (pH 6) to investigate the desorption, but no desorption was found. The maximum immobilization capacities were found to be 90 µg protein cm−2 film in optimum conditions.

Index Entries

Yeast cell immobilization chitosan 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Seki, M., Naito, K. I., and Frusaki, S. (1993), J. Chem. Eng. Japan 26, 662–668.CrossRefGoogle Scholar
  2. 2.
    Aykut, G., Hasirci, V. N., and Alaeddinoğlu, G. (1988), Biomaterials 9, 168–172.CrossRefGoogle Scholar
  3. 3.
    Gong, H. P., Zong, Y. H., Li, J. H., Gong, Y. D., Zhao, N. M., and Zhang, X. F. (2000), J. Biomed. Mater. Res. 52, 285–295.CrossRefGoogle Scholar
  4. 4.
    Kumar, M. N. V. R. (2000), React. Func. Polym. 46, 1–27.CrossRefGoogle Scholar
  5. 5.
    Sabnis, S. and Block, L. H. (2000), Int. J. Biol. Macromol. 27, 181–186.CrossRefGoogle Scholar
  6. 6.
    Oztop, H. N., Oztop, A. Y., Isikver, Y., and Saraydin, D. (2002), Proc. Biochem. 37, 651–657.CrossRefGoogle Scholar
  7. 7.
    Bardi, E. P., Koutinas A. A., Soupioni, M. J., and Kanellaki, M. E. (1996), J. Agric. Food Chem. 44, 463–467.CrossRefGoogle Scholar
  8. 8.
    Xin, L. Z., Carenza, M., Kaetsu, I., Kumakura, M., Yoshida, M., and Fujimura, T. (1992), Radiat. Phys. Chem. 40, 579–584.Google Scholar
  9. 9.
    Prakasham, R. S., Kuriakose, B., and Ramakhrisna, S. V. (1999), App. Biochem. Biotechnol. 82, 127–134.CrossRefGoogle Scholar
  10. 10.
    Paul, W. and Sharma, C. P. (2000), S. T. P. Pharm. Sci. 10, 5–22.Google Scholar
  11. 11.
    Yamamoto, A., Tozaki, H., Okada, N., and Fujita, T. (2000), S. T. P. Pharm. Sci. 10, 23–34.Google Scholar
  12. 12.
    Long, D. D. and Luyen, D. V. (1996), J. M. S. Pure Appl. Chem. A33, 1975–1884.Google Scholar
  13. 13.
    Çetinus, S. A. and Öztop, H. N. (2000), Enzyme and Microbial Technol. 26, 497–501.CrossRefGoogle Scholar
  14. 14.
    Scopes, K. R. (1984), Protein Purification Principles Practices, Springer-Verlag, NY, pp. 1–266.Google Scholar
  15. 15.
    Yao, K. D., Peng, T., Goosen, M. F. A., Min, J. M., and He, Y. Y. (1993), J. Appl. Polym. Sci. 48, 343–354.CrossRefGoogle Scholar
  16. 16.
    Tual, C., Espuche, E., Escoubes, M., and Donart, A. (2000), J. Polym. Sci. Part B-Polym. Phys. 38, 1521–1529.CrossRefGoogle Scholar
  17. 17.
    Gupta, K. C. and Kumar, M. N. V. R. (2000), Biomaterials 21, 1115–1119.CrossRefGoogle Scholar
  18. 18.
    Mi, F. I., Shyu, S. S., Wu, Y. B., Lee, S. T., Shyong, J. Y., and Huang, R. N. (2001), Biomaterials 22, 165–173.CrossRefGoogle Scholar
  19. 19.
    Saraydin, D., Öztop, H. N., Karadağ, E., Çaldiran, Y., and Güven, O. (1999), Appl. Biochem. Biotechnol. 82, 115–125.CrossRefGoogle Scholar
  20. 20.
    Saraydin, D., Karadağ, E. Sahiner, N., and Güven, O. (2001), J. Macromol. Sci. Pure Appl. Chem. A38, 1105–1121.Google Scholar
  21. 21.
    Öztop, H. N., Saraydin, D., Karadağ, E., Çaldiran, Y., and Güven, O. (1999), Polym. Bull. 40, 575–581.Google Scholar

Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  • H. Nursevin Öztop
    • 1
  • Dursun Saraydin
    • 2
  • Şenay Cetinus
    • 1
  1. 1.Department of Chemistry, Biochemistry Research LaboratoryCumhuriyet UniversitySivasTurkey
  2. 2.Department of Chemistry, Hydrogel Research LaboratoryCumhuriyet UniversitySivasTurkey

Personalised recommendations