Applied Biochemistry and Biotechnology

, Volume 101, Issue 3, pp 239–249 | Cite as

pH-sensitive chitosan films for baker’s yeast immobilization

  • H. Nursevin Öztop
  • Dursun Saraydin
  • Şenay Cetinus
Article

Abstract

Dried baker’s yeast cells were immobilized on a chitosan film, which is a natural polymer. Prepared chitosan films were treated with glutaraldehyde to facilitate the immobilization of the cells. The effects of the amount of glutaraldehyde, incubation time, pH, and temperature on immobilization were investigated. The amount of glutaraldehyde was chosen to be 0.01% (weight). The highest amount of yeast immobilization was obtained with 5 h incubation. It was determined that optimum temperature for immobilization is 25°C, and the optimum pH for immobilization is 6. Immobilized cells were allowed to stand for 3 d in distilled water and buffer solution (pH 6) to investigate the desorption, but no desorption was found. The maximum immobilization capacities were found to be 90 µg protein cm−2 film in optimum conditions.

Index Entries

Yeast cell immobilization chitosan 

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Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  • H. Nursevin Öztop
    • 1
  • Dursun Saraydin
    • 2
  • Şenay Cetinus
    • 1
  1. 1.Department of Chemistry, Biochemistry Research LaboratoryCumhuriyet UniversitySivasTurkey
  2. 2.Department of Chemistry, Hydrogel Research LaboratoryCumhuriyet UniversitySivasTurkey

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