Abstract
A simultaneous separation of four metal ions of Cd2+, Co2+, Zn2+, and Cu2+ was developed by capillary electrophoresis by using HQ3 (Bis(2-pyridylmethyl)(8-pyridylmethyloxy-quinoline-2-methyl) amine) as chelating ligand. It has been demonstrated that HQ3 plays two important roles in the simultaneous separation by CE. The first role is to improve separation selectivity of Zn2+, Cd2+, Co2+, and Cu2+ by forming metal-HQ3 complexes. Another is to make the UV detection of metal ions possible with high UV sensitivity. The separation conditions have been optimized. The baseline separation of Zn2+, Cd2+, Co2+, and Cu2+ has been carried out by using running buffer of 60 mM acetate buffer and 2 mM cetyltrimethyl ammonium bromide (CTAB) at pH 4.0. The separation behaviors including interference of other metal ions, effect of buffer pH, buffer concentration, CTAB concentration have been examined. The method has been validated and applied to determine metal ions in serum sample.
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Acknowledgments
This work was supported by the start-up fund for Luojia Chair Professorship of Wuhan University (No. 306276216) and partially by the National Scientific Foundation of China (NSFC Nos. 90817103, 30973672 and No. 20775055), the Fundamental Research Funds for the Central Universities and Important National Science & Technology Specific Projects (No. 2009ZX09301-14).
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Zhang, W., Wu, Y., Chen, Y. et al. Simultaneous Separation of Four Metal Ions by CE Using Bis(2-Pyridylmethyl) (8-Pyridylmethyloxy-Quinoline-2-Methyl)Amine as Chelating Agent. Chroma 72, 1201–1205 (2010). https://doi.org/10.1365/s10337-010-1775-7
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DOI: https://doi.org/10.1365/s10337-010-1775-7